This study aimed to determine whether actinomycetes could contribute to the odours occurring in Lake Lotus, China. In total, 55 actinomycete strains were isolated from sediments in Lake Lotus and their odorous metabolites, geosmin and 2-methylisoborneol (2-MIB), were identified using HSPME-GC-MS. Results showed that 24, 23 and 34 strains produced geosmin and/or 2-MIB in Gause, TSB and M liquid media, respectively. Of odour-producing actinomycetes, most could produce geosmin and some produced both metabolites, while few of them produced only 2-MIB. Six isolates with high-level odour were selected for further investigation. Their biomass and odour-producing abilities were monitored in both the slants and liquid media. The results suggest that TSB was the most suitable medium for the growth of mycelium; Gause and M slant supported good production of spores, while M liquid medium was the most favourable for the production of both geosmin and 2-MIB. Those strains that produced geosmin only were less influenced than those that produced both geosmin and 2-MIB under shaking conditions. The results indicate that actinomycetes from sediments should be taken into consideration when off-flavours occur in water columns. According to the 16S rRNA sequences, six actinomycetes were classified in the Streptomyces.
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December 2009
This article was originally published in
Journal of Water Supply: Research and Technology-Aqua
Article Contents
Research Article|
December 01 2009
Isolation, identification and odour-producing abilities of geosmin/2-MIB in actinomycetes from sediments in Lake Lotus, China
Yanxia Zuo;
Yanxia Zuo
1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China
2Graduate School of the Chinese Academy of Science, Beijing 100039, China
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Lin Li;
1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China
E-mail: [email protected]
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Zongxing Wu;
Zongxing Wu
1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China
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Lirong Song
1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China
E-mail: [email protected]
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Journal of Water Supply: Research and Technology-Aqua (2009) 58 (8): 552–561.
Article history
Received:
January 15 2009
Accepted:
August 25 2009
Citation
Yanxia Zuo, Lin Li, Zongxing Wu, Lirong Song; Isolation, identification and odour-producing abilities of geosmin/2-MIB in actinomycetes from sediments in Lake Lotus, China. Journal of Water Supply: Research and Technology-Aqua 1 December 2009; 58 (8): 552–561. doi: https://doi.org/10.2166/aqua.2009.018
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