Pilot-scale biofilters treated with real groundwater were established to systemically investigate the interactions between ammonia, iron and manganese removal. When both of ammonia and manganese in influent were about 1 mg/L, they were quickly removed; but when manganese was above 3 mg/L, ammonia removal was affected significantly. When total iron was above 5 mg/L, the oxidization rate of ammonia decreased significantly. In addition, manganese and iron removal were not affected by ammonia, when ammonia was lower than 2.5 mg/L. Fe2+ could react with manganese oxides in the biofilter, thus manganese removal could only take place after Fe2+ was completely oxidized. When total iron, manganese and ammonia in influent were 11.27 mg/L, 1.20 mg/L and 1.27 mg/L, respectively, iron, ammonia and manganese were mainly removed in 0–0.3 m, 0–0.4 m and 0.2–0.8 m of the filter depth, respectively, and nitrite oxidizing bacteria were presented in 0–0.3 m of the filter depth. The pH decreased along the filter depth with the oxidation of ammonia, iron and manganese, while oxidation reduction potential increased. The results of this study were useful for the optimization and design of biofilters.
INTRODUCTION
Groundwaters are often mildly acidic and devoid of dissolved oxygen (DO) (Azher et al. 2008), therefore soluble Fe2+ and Mn2+ are usually present in groundwaters by contacting with rocks and minerals, either in dissolved mineral forms, Fe2+ and Mn2+, or associated with various organics, minerals or chelating agents (Jusoh et al. 2005), especially in Northeast China (Li et al. 2013). A suitable water supply for people and industry has currently become more difficult (Okoniewska et al. 2007), because intensive development of industry branches and agriculture lead to fast degradation of the environment and simultaneously to pollution of drinking water reservoirs. Both in surface waters and groundwaters, an increase in concentration of different elements and compounds has been observed in the past years (Kabata-Pendias & Pendias 1993). The contaminations, which come from industrial or urban wastewater, wastes, sludge, pesticides and artificial fertilizers, and their chemical compositions, that are mainly influenced by the content of industrial organic compounds, affect organoleptic properties of water, are toxic or even carcinogenic for human beings (Zaw & Chiswell 1999).
Groundwater as a reservoir for water supply systems usually contains high concentrations of iron and manganese; at the same time, a continuously increasing ammonia concentration in groundwater has been observed in the past several years (Okoniewska et al. 2007). The presence of ammonia, iron and manganese in drinking water should be avoided and the maximum contaminant levels (MCLs) for ammonia of 0.5 mg/L, total iron of 0.3 mg/L and manganese of 0.1 mg/L have been established in China (GB 5749–2006). Iron and manganese are objectionable for the following reasons. (a) Iron and manganese give water a reddish and brown-black colour, respectively, when exposed to air; furthermore, their precipitates can cause stains at laundry and sanitary facilities (Azher et al. 2005, 2008; Tekerlekopoulou & Vayenas 2008). (b) Iron and manganese deposits build up in pipelines, reduce pipe diameter in distribution systems and eventually clog pipes, or build up in water heaters and weaken the heating efficiency. (c) Iron and manganese are substrates for the growth of bacteria in drains and water distribution systems (Azher et al. 2005); therefore, bad odours and unpleasant tastes may be caused by by-products of the bacteria growth and the dead bacteria (Gouzinis et al. 1998). In addition, when manganese exceeds the MCLs, it has been found to affect the central nervous system (Sharma et al. 2001). The presence of ammonia in drinking water treatment could affect the chlorination process (Tekerlekopoulou & Vayenas 2007; Hasan et al. 2013). This is because ammonia can react with chlorine to form disinfection by-products (Charrois & Hrudey 2007), which could damage the human nervous system and cause a deterioratation in the taste and odour of water (Richardson et al. 2007), and also reduce disinfection efficiency (WHO 1996). Moreover, ammonia can interfere with the manganese biofiltration process by consuming excessive oxygen during nitrification, resulting in mouldy and earthy tasting water (WHO 1996).
Chemical methods can be used to oxidize ammonia, iron and manganese, but may produce potential hazardous by-products. As well, they may introduce other pollutants into the produced water (Han et al. 2013). Thus, the biological removal of ammonia, iron and manganese emerged and has gradually replaced the conventional chemical treatments (Tekerlekopoulou et al. 2013). However, it has been reported that achieving simultaneous removal of ammonia and manganese is very difficult (Pacini et al. 2005; Han et al. 2013), since biological manganese removal can only take place after complete nitrification because of the necessary evolution of the oxidation reduction potential (ORP) (Hasan et al. 2012). In addition, manganese removal only takes place after Fe2+ is completely oxidized. Although the interactions between ammonia, iron and manganese have been investigated in previous studies (Gouzinis et al. 1998; Tekerlekopoulou & Vayenas 2007, 2008) and some experimental results obtained, few researchers have systematically investigated the interactions between ammonia, iron and manganese, to know how and why ammonia, iron or manganese influence each other in the biofilter, especially when the concentrations of the pollutants were all relatively high.
In this study, pilot-scale biofilters were established for ammonia, iron and manganese removal using manganese sand as the media. In contrast to all previous reports, real feed groundwater was selected to carry out this experiment because polluted groundwater is becoming an important international issue, especially for developing countries. An approximately 200 day long-term operation was evaluated with respect to the interactions between ammonia and manganese, ammonia and iron, and manganese and iron, and the interactions among ammonia, iron and manganese in a biofilter. The main objectives of this study were to gain an in-depth insight into the interactions between ammonia, iron and manganese in the biofilters to know how and why ammonia, iron or manganese influence each other, and how ammonia, iron and manganese were removed in a biofilter, and thus to provide guidance for the optimization and design of biofilters.
MATERIALS AND METHODS
Groundwater, which was extracted from wells with a depth of 40–50 m, in Harbin city, P. R. China, was used throughout this experiment. The concentration of DO, total iron, manganese and ammonia in raw groundwater was 0.15–0.25 mg/L, 9–12 mg/L, 0.9–1.3 mg/L and 0.9–1.4 mg/L, respectively. The concentration of CODMn in influent and effluent was 2.14 mg/L and 1.08 mg/L, respectively. The temperature was 7.8–8.0 °C, and pH was 6.9–7.10. In order to increase the concentration of ammonia, iron and manganese in influent, stock solutions of 10 g/L , 10 g/L Fe(II) and 10 g/L Mn(II) were prepared by diluting NH4Cl, FeSO4·7H2O and MnSO4·H2O, respectively.
Water sampling from the inlet and outlet was performed every day and water sampling along the height of the filter bed was performed twice a week. To make sure the operation was stable, the biofilter was operated for about 15 days after changing influent concentration. It should be noted that all biofilters operated for about one year before this experiment.
Ammonia and manganese removal
Two pilot-scale biofilters were constructed for simultaneous removal of ammonia and manganese (Figure 1(a)); the internal diameters of filters 1 and 2 were 250 mm and 150 mm, respectively. First, raw water was sprayed into tank 1 and the DO increased to 8.0 mg/L, and then it was treated in filter 1. Next, the aerated effluent water of filter 1 in tank 2 with a DO of 11 mg/L was added to filter 2 with manganese and ammonia solutions in tanks 3 and 4, respectively. In order to investigate the influence of varying manganese concentrations on ammonia removal, the concentrations of manganese in influent was increased from about 1 to 4 mg/L, and ammonia was about 1 mg/L. The influence of varying ammonia concentrations on manganese removal was also investigated in the same way; ammonia in influent increased from about 1.5 to 2.5 mg/L, and manganese was about 1 mg/L.
Ammonia and iron removal
This experiment was operated as in the previous section except that an iron solution was prepared in tank 3. In order to investigate the influence of varying iron concentrations on ammonia removal, the concentration of total iron in influent was increased from about 0.1 to 10 mg/L, and ammonia about 1 mg/L. Then, the influence of varying ammonia concentrations on iron removal was also investigated; ammonia in influent increased from about 1.5 to 2.5 mg/L and total iron was about 10 mg/L.
Manganese and iron removal
A pilot-scale biofilter with an internal diameter of 150 mm was used for simultaneous removal of manganese and iron (Figure 1(b)). Raw water flowed to the sealed tank 5, and then was pumped to the mixing chamber of filter 3, and finally flowed to the filter. DO in the raw water increased to 3.2 mg/L after that process. The concentration profiles of total iron and manganese along the filter depth were measured to investigate the efficiency of iron and manganese removal, when DO was insufficient to completely oxidize ferric iron (part of DO was utilized to oxidize ammonia).
Ammonia, iron and manganese removal
This experiment was done as in the previous section, except that raw water was sprayed into tank 6 (Figure 1(c)). The concentration profiles of ammonia, iron and manganese along the filter depth were measured twice a week to investigate simultaneous removal of ammonia, iron and manganese in the biofilter.
The presence of microorganism and nitrite oxidizing bacteria (NOB) in depths of 0 m, 0.1 m, 0.2 m and 0.3 m of filter 4 were detected by applying the molecular characterization method of fluorescence in situ hybridization (FISH) (Wagner et al. 1996). 4′-6-diamidino-2-phenylindole (DAPI) probe was used to target all of the microorganisms in the biofilms which revealed a significant distribution, and Nit 3 (Cy3 40 CCTGTGCTCCATGCTCCG) probe was used to target NOB groups.
Analysis methods
The pH, ORP and DO measurements were conducted using a pH meter (Ultra BASIC UB-10), an ORP meter (pH 315i-WTW) and a DO meter (Oxi 315i-WTW), respectively. Total iron, manganese and ammonia in water samples were measured by photometric method, according to Standard Methods for the Examination of Water and Wastewater (Method Nos 3500-Fe.B, 3500-Mn.B and 4500-NH3.B and C, respectively) (Eaton et al. 2005).
RESULTS AND DISCUSSION
Ammonia and manganese removal
When the concentration of manganese and ammonia in the influent of filter 2 was 0.93 mg/L and 1.16 mg/L (Table 1), respectively, manganese and ammonia decreased to 0.02 mg/L and 0.03 mg/L in 0.1 m of the filter depth, respectively, meaning manganese and ammonia could be simultaneously removed in the biofilter. When manganese increased to about 2 mg/L, 3 mg/L and 4 mg/L, manganese in 0.1 m increased to 0.07 mg/L, 0.47 mg/L and 1.40 mg/L, respectively; surprisingly, ammonia in 0.1 m increased to 0.06 mg/L, 0.17 mg/L and 0.35 mg/L, respectively, although ammonia in influent was still about 1 mg/L. Ammonia removal was affected significantly, when manganese was higher than 3 mg/L. When manganese increased to about 3 mg/L or 4 mg/L, manganese increased along the filter depth in the first day (data not shown) while ammonia did not increase; then manganese decreased in the next few days, but ammonia increased. Thus, it may be concluded, that when manganese in influent increased to 3 mg/L or even higher, more manganese oxides were produced and covered the support materials’ surface, which influenced the results of ammonia removal.
Manganese concentration profiles along the filter depth for feed concentration of about 1.0 mg/L, 2.0 mg/L, 3.0 mg/L and 4.0 mg/L, respectively, when ammonia in influent was about 1 mg/L
Filter depth (m) . | Mn 1.0 (mg/L) . | Mn 2.0 (mg/L) . | Mn 3.0 (mg/L) . | Mn 4.0 (mg/L) . | ||||
---|---|---|---|---|---|---|---|---|
Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | |
0 | 0.93 | 1.16 | 1.98 | 1.07 | 2.93 | 1.13 | 4.24 | 1.12 |
0.1 | 0.02 | 0.03 | 0.07 | 0.06 | 0.47 | 0.17 | 1.40 | 0.35 |
0.2 | 0.00 | 0.03 | 0.00 | 0.05 | 0.09 | 0.08 | 0.34 | 0.10 |
0.3 | 0.00 | 0.03 | 0.00 | 0.04 | 0.00 | 0.06 | 0.00 | 0.09 |
0.4 | 0.00 | 0.02 | 0.00 | 0.03 | 0.00 | 0.04 | 0.00 | 0.07 |
0.8 | 0.00 | 0.03 | 0.00 | 0.03 | 0.00 | 0.03 | 0.00 | 0.04 |
1.2 | 0.00 | 0.03 | 0.00 | 0.02 | 0.00 | 0.03 | 0.00 | 0.03 |
1.5 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.01 |
Filter depth (m) . | Mn 1.0 (mg/L) . | Mn 2.0 (mg/L) . | Mn 3.0 (mg/L) . | Mn 4.0 (mg/L) . | ||||
---|---|---|---|---|---|---|---|---|
Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | |
0 | 0.93 | 1.16 | 1.98 | 1.07 | 2.93 | 1.13 | 4.24 | 1.12 |
0.1 | 0.02 | 0.03 | 0.07 | 0.06 | 0.47 | 0.17 | 1.40 | 0.35 |
0.2 | 0.00 | 0.03 | 0.00 | 0.05 | 0.09 | 0.08 | 0.34 | 0.10 |
0.3 | 0.00 | 0.03 | 0.00 | 0.04 | 0.00 | 0.06 | 0.00 | 0.09 |
0.4 | 0.00 | 0.02 | 0.00 | 0.03 | 0.00 | 0.04 | 0.00 | 0.07 |
0.8 | 0.00 | 0.03 | 0.00 | 0.03 | 0.00 | 0.03 | 0.00 | 0.04 |
1.2 | 0.00 | 0.03 | 0.00 | 0.02 | 0.00 | 0.03 | 0.00 | 0.03 |
1.5 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.01 |
When the concentration of manganese in influent was about 1 mg/L and ammonia increased to about 1.5 mg/L, 2 mg/L and 2.5 mg/L (Table 2), respectively, ammonia slightly increased along the filter depth, but manganese concentrations hardly varied. The varying concentrations of ammonia did not affect manganese removal. Tekerlekopoulou & Vayenas (2008) investigated the interactions among ammonia, iron and manganese removal when simultaneously present in the trickling filter, and found that ammonia drastically affected manganese oxidation, which was not in agreement with our results. In our previous experiments, it was also found that manganese oxidation was affected significantly by ammonia, when nitrite in high concentrations accumulated in the biofilter; but when nitrite was oxidized to nitrate, manganese oxidation was not affected by ammonia (Cheng et al. 2016). In this experiment, there were plenty of NOB in the biofilter, which could quickly oxidize nitrite to nitrate, and the data were measured in steady phase.
Ammonia concentration profiles along the filter depth for feed concentration of about 1.5 mg/L, 2.0 mg/L and 2.5 mg/L, respectively, when manganese in influent was about 1 mg/L
Filter depth (m) . | NH4+-N 1.5 (mg/L) . | NH4+-N 2.0 (mg/L) . | NH4+-N 2.5 (mg/L) . | |||
---|---|---|---|---|---|---|
NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | |
0 | 1.57 | 1.11 | 1.99 | 0.90 | 2.43 | 1.04 |
0.1 | 0.09 | 0.05 | 0.17 | 0.02 | 0.25 | 0.02 |
0.2 | 0.06 | 0.00 | 0.12 | 0.00 | 0.18 | 0.00 |
0.3 | 0.05 | 0.00 | 0.09 | 0.00 | 0.12 | 0.00 |
0.4 | 0.04 | 0.00 | 0.08 | 0.00 | 0.09 | 0.00 |
0.8 | 0.03 | 0.00 | 0.07 | 0.00 | 0.06 | 0.00 |
1.2 | 0.02 | 0.00 | 0.06 | 0.00 | 0.04 | 0.00 |
1.5 | 0.02 | 0.00 | 0.03 | 0.00 | 0.04 | 0.00 |
Filter depth (m) . | NH4+-N 1.5 (mg/L) . | NH4+-N 2.0 (mg/L) . | NH4+-N 2.5 (mg/L) . | |||
---|---|---|---|---|---|---|
NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | NH4+-N (mg/L) . | Mn (mg/L) . | |
0 | 1.57 | 1.11 | 1.99 | 0.90 | 2.43 | 1.04 |
0.1 | 0.09 | 0.05 | 0.17 | 0.02 | 0.25 | 0.02 |
0.2 | 0.06 | 0.00 | 0.12 | 0.00 | 0.18 | 0.00 |
0.3 | 0.05 | 0.00 | 0.09 | 0.00 | 0.12 | 0.00 |
0.4 | 0.04 | 0.00 | 0.08 | 0.00 | 0.09 | 0.00 |
0.8 | 0.03 | 0.00 | 0.07 | 0.00 | 0.06 | 0.00 |
1.2 | 0.02 | 0.00 | 0.06 | 0.00 | 0.04 | 0.00 |
1.5 | 0.02 | 0.00 | 0.03 | 0.00 | 0.04 | 0.00 |
Ammonia and iron removal
When the concentration of total iron and ammonia in influent was 0.11 mg/L and 1.30 mg/L, respectively, ammonia concentration decreased to 0.12 mg/L in 0.1 m of the filter depth (Table 3). However, when total iron increased to 5.37 mg/L and 11.27 mg/L, ammonia increased to 0.50 mg/L and 0.77 mg/L in 0.1 m, respectively, and then dropped to 0.081 mg/L and 0.10 mg/L in 0.4 m, respectively; the removal rate of ammonia decreased significantly with the increase of iron content. This was because when total iron was higher than 5 mg/L the oxidized ferric hydroxide covered the support materials’ surface and prevented ammonia oxidizing bacteria (AOB) and NOB contacting with ammonia in the fluid, which thus affected the growth of AOB and NOB. Total iron increased along the filter depth with the increase of total iron concentration in influent.
Total iron concentration profiles along the filter depth for feed concentration of about 0.1 mg/L, 5.0 mg/L and 10 mg/L, respectively, when ammonia in influent was about 1 mg/L
Filter depth (m) . | Total Fe 0.1 (mg/L) . | Total Fe 5 (mg/L) . | Total Fe 10 (mg/L) . | |||
---|---|---|---|---|---|---|
Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | |
0 | 0.11 | 1. 30 | 5.37 | 1.13 | 11.27 | 1.27 |
0.1 | 0.068 | 0.12 | 1.02 | 0.50 | 2.28 | 0.77 |
0.2 | 0.048 | 0.097 | 0.35 | 0.24 | 0.67 | 0.44 |
0.3 | 0.034 | 0.070 | 0.18 | 0.12 | 0.33 | 0.22 |
0.4 | 0.024 | 0.053 | 0.068 | 0.081 | 0.097 | 0.10 |
0.8 | 0.013 | 0.043 | 0.018 | 0.059 | 0.019 | 0.075 |
1.2 | 0.0056 | 0.032 | 0.0075 | 0.043 | 0.0095 | 0.053 |
1.5 | 0.0027 | 0.015 | 0.0036 | 0.037 | 0.0046 | 0.043 |
Filter depth (m) . | Total Fe 0.1 (mg/L) . | Total Fe 5 (mg/L) . | Total Fe 10 (mg/L) . | |||
---|---|---|---|---|---|---|
Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | |
0 | 0.11 | 1. 30 | 5.37 | 1.13 | 11.27 | 1.27 |
0.1 | 0.068 | 0.12 | 1.02 | 0.50 | 2.28 | 0.77 |
0.2 | 0.048 | 0.097 | 0.35 | 0.24 | 0.67 | 0.44 |
0.3 | 0.034 | 0.070 | 0.18 | 0.12 | 0.33 | 0.22 |
0.4 | 0.024 | 0.053 | 0.068 | 0.081 | 0.097 | 0.10 |
0.8 | 0.013 | 0.043 | 0.018 | 0.059 | 0.019 | 0.075 |
1.2 | 0.0056 | 0.032 | 0.0075 | 0.043 | 0.0095 | 0.053 |
1.5 | 0.0027 | 0.015 | 0.0036 | 0.037 | 0.0046 | 0.043 |
When the concentration of total iron in influent was about 9 mg/L and ammonia was about 1.5 mg/L, 2.0 mg/L and 2.5 mg/L (Table 4), respectively, ammonia increased along the filter depth with the increase of ammonia in influent, while total iron hardly varied along the filter depth. When ammonia in influent was about 2.5 mg/L, ammonia was not completely removed because there was an insufficient amount of DO. The varying concentration of ammonia hardly affected iron removal when ammonia was lower than 2.5 mg/L.
Ammonia concentration profiles along the filter depth for feed concentration of about 1.5 mg/L, 2.0 mg/L and 2.5 mg/L, respectively, when total Fe in influent was about 9 mg/L
Filter depth (m) . | NH4+-N 1.5 (mg/L) . | NH4+-N 2 (mg/L) . | NH4+-N 2.5 (mg/L) . | |||
---|---|---|---|---|---|---|
NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | |
0 | 1.56 | 9.38 | 1.99 | 9.05 | 2.42 | 9.23 |
0.1 | 1.09 | 1.95 | 1.43 | 2.09 | 1.93 | 2.00 |
0.2 | 0.75 | 0.79 | 1.08 | 0.91 | 1.59 | 0.84 |
0.3 | 0.45 | 0.44 | 0.54 | 0.38 | 0.86 | 0.39 |
0.4 | 0.13 | 0.10 | 0.17 | 0.05 | 0.62 | 0.07 |
0.8 | 0.10 | 0.03 | 0.12 | 0.03 | 0.56 | 0.05 |
1.2 | 0.08 | 0.02 | 0.10 | 0.02 | 0.53 | 0.04 |
1.5 | 0.04 | 0.01 | 0.08 | 0.01 | 0.49 | 0.02 |
Filter depth (m) . | NH4+-N 1.5 (mg/L) . | NH4+-N 2 (mg/L) . | NH4+-N 2.5 (mg/L) . | |||
---|---|---|---|---|---|---|
NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | NH4+-N (mg/L) . | Total Fe (mg/L) . | |
0 | 1.56 | 9.38 | 1.99 | 9.05 | 2.42 | 9.23 |
0.1 | 1.09 | 1.95 | 1.43 | 2.09 | 1.93 | 2.00 |
0.2 | 0.75 | 0.79 | 1.08 | 0.91 | 1.59 | 0.84 |
0.3 | 0.45 | 0.44 | 0.54 | 0.38 | 0.86 | 0.39 |
0.4 | 0.13 | 0.10 | 0.17 | 0.05 | 0.62 | 0.07 |
0.8 | 0.10 | 0.03 | 0.12 | 0.03 | 0.56 | 0.05 |
1.2 | 0.08 | 0.02 | 0.10 | 0.02 | 0.53 | 0.04 |
1.5 | 0.04 | 0.01 | 0.08 | 0.01 | 0.49 | 0.02 |
Manganese and iron removal
Total iron and manganese concentration profiles along the filter depth, when DO in influent was 3.2 mg/L.
Ammonia, iron and manganese removal
Total iron, manganese and ammonia concentration profiles along filter 4 depth.
Molecular characterization of the microbial community by FISH method, other broader bacterial groups characterized by DAPI and NOB in biofilms clumped characterized by FITC. (a) 0 m, (b) 0.1 m, (c) 0.2 m and (d) 0.3 m of filter 4 depth.
CONCLUSIONS
Ammonia and manganese, and ammonia and iron could be simultaneously removed in the biofilter, but when manganese and iron in influent were above 3 mg/L and 5 mg/L, respectively, the removal rates of ammonia decreased significantly. Iron and manganese removal was not affected by ammonia, when ammonia was lower than 2.5 mg/L. Manganese removal occurred after Fe2+ was completely oxidized, since Fe2+ could react with manganese oxides. In the biofilter for ammonia, iron and manganese removal, ammonia and iron were simultaneously removed from the top of the filter depth, and manganese removal occurred when Fe2+ was completely oxidized. The pH decreased along the filter depth, while ORP increased.
ACKNOWLEDGEMENTS
This work was kindly supported by the Scientific Research Foundation of Chengdu University of Information Technology (CUIT) (KYTZ201511) and Program of Education Department of Sichuan Province (16ZB0221).