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Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures

By
Paul Monis
Paul Monis
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Nic Reid
Nic Reid
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IWA Publishing
Volume
14
ISBN electronic:
9781780407524
Publication date:
May 2015

Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP's developed.

This book is co-published with Water Research Australia

This title belongs to WaterRA

ISBN: 9781780407524 (eBook)

ISBN: 9781780407517 (Print)

Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures
By: Paul Monis, Nic Reid
DOI: https://doi.org/10.2166/9781780407524
ISBN (electronic): 9781780407524
Publisher: IWA Publishing
Published: 2015

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