Dividing a water distribution network (WDN) into district metered areas (DMAs) is the first vital step towards pressure management and real losses reduction. However, other factors of water quality such as the water age must be taken into account while forming DMAs. The current study uses genetic algorithm (GA) optimization methods to achieve the desired WDN segmentation conditions in terms of: (a) reducing the operating pressure, thus reducing the system's real losses; and (b) reducing the water age, thus improving the feeling of water freshness and preventing growth of disinfection byproducts. Techniques based on GA are a proven way to provide a very good solution to optimization problems. The solution is obtained using an objective function and setting boundary constraints. The formation of the objective functions is tested through Matlab's optimization toolbox. The logic of the objective functions' formulation for both the operating pressure and the water age optimization is recorded and analyzed. The method's application utilized a sample network model assisted by EPANET and Bentley's WaterGEMS software tools. The morphology of the DMAs is presented for each scenario, as well as the results of the network's segmentation according to the operating pressure and the water age.