To the best of our knowledge, there was little information available on pathogen removal using low level disinfectant followed by free chlorine in sequential disinfection (SD). This study investigated Escherichia coli inactivation by four types of disinfection: single step disinfection (SSD), SD, traditional sequential disinfection (TSD) and mixed disinfectant disinfection (MDD). Results indicated that SD had higher ability to inactivate E. coli than the others, indicating there was a positive synergistic effect on chlorine disinfection by prior dosing with a low level of chlorine dioxide (ClO2). The ONPG assay suggested that the permeability of cell wall rather than the viability of E. coli were changed under 0.02 mg/l ClO2 treatment. The coexistence of residual ClO2 and free chlorine also plays an active synergistic effect. Additionally, temperature had a positive effect on E. coli inactivation in SD, while inactivation was reduced in alkaline compared to neutral and acidic conditions.
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Research Article|
October 18 2012
The synergistic effect of Escherichia coli inactivation by sequential disinfection with low level chlorine dioxide followed by free chlorine
Wu Yang;
Wu Yang
1School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China
2Department of Environment and Health, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin, 300050, China
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Dong Yang;
Dong Yang
2Department of Environment and Health, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin, 300050, China
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Sui-Yi Zhu;
Sui-Yi Zhu
1School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China
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Bo-Yan Chen;
Bo-Yan Chen
3The College of Environmental Science and Engineering, Nankai University, Tianjin, 300192, China
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Ming-Xin Huo;
Ming-Xin Huo
1School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China
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Jun-Wen Li
2Department of Environment and Health, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin, 300050, China
E-mail: [email protected]
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J Water Health (2012) 10 (4): 557–564.
Article history
Received:
April 23 2012
Accepted:
September 04 2012
Citation
Wu Yang, Dong Yang, Sui-Yi Zhu, Bo-Yan Chen, Ming-Xin Huo, Jun-Wen Li; The synergistic effect of Escherichia coli inactivation by sequential disinfection with low level chlorine dioxide followed by free chlorine. J Water Health 1 December 2012; 10 (4): 557–564. doi: https://doi.org/10.2166/wh.2012.067
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