The response of a representative virus and indicator bacteria to heating, solar irradiation, or their combination, was investigated in a controlled solar simulator and under real sun conditions. Heating showed higher inactivation of Escherichia coli compared to the bacteriophage MS2. Heating combined with natural or simulated solar irradiation demonstrated a synergistic effect on the inactivation of E. coli, with up to 3-log difference for 50 °C and natural sun insolation of 2,000 kJ m−2 (compared to the sum of the separate treatments). Similar synergistic effect was also evident when solar-UV induced DNA damage to E. coli was assessed using the endonuclease sensitive site assay (ESS). MS2 was found to be highly resistant to irradiation and heat, with a slightly synergistic effect observed only at 59 °C and natural sun insolation of 5,580 kJ m−2. Heat treatment also hindered light-dependent recovery of E. coli making the treatment much more effective.
Synergistic effect of heat and solar UV on DNA damage and water disinfection of E. coli and bacteriophage MS2
Dana Jennifer Theitler, Abid Nasser, Yoram Gerchman, Abraham Kribus, Hadas Mamane; Synergistic effect of heat and solar UV on DNA damage and water disinfection of E. coli and bacteriophage MS2. J Water Health 1 December 2012; 10 (4): 605–618. doi: https://doi.org/10.2166/wh.2012.072
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