The antibacterial activity of a water-soluble chitosan derivative prepared by chemical modification to quaternary ammonium compound N,N,N-trimethylchitosan (TC) was investigated against four selected waterborne pathogens: Aeromonas hydrophila ATCC 35654, Yersinia enterocolitica ATCC 9610, Listeria monocytogenes ATCC 19111 and Escherichia coli O157:H7 ATCC 32150. An inactivation of 4 log CFU/ml of all waterborne pathogens was noted for the quaternized chitosan as compared with chitosan over a short contact time (30 min) and low dosage (4.5 ppm) at ambient temperature. A marked increase in glucose level, protein content and lactate dehydrogenase (LDH) activity was observed concurrently in the cell supernatant to be a major bactericidal mechanism. The results suggest that the TC derivative may be a promising commercial substitute for acid-soluble chitosan for rapid and effective disinfection of water.
Development of a quaternized chitosan with enhanced antibacterial efficacy
Gurpreet Kaur Khaira, Rashmi Kumariya, Manmohan Chibber, Moushumi Ghosh; Development of a quaternized chitosan with enhanced antibacterial efficacy. J Water Health 1 September 2013; 11 (3): 410–418. doi: https://doi.org/10.2166/wh.2013.029
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