We previously demonstrated the colonization of Mycobacterium avium complex in bathrooms by the conventional culture method. In the present study, we aimed to directly detect M. avium organisms in the environment using loop-mediated isothermal amplification (LAMP), and to demonstrate the efficacy of LAMP by comparing the results with those obtained by culture. Our data showed that LAMP analysis has detection limits of 100 fg DNA/reaction for M. avium. Using an FTA® elute card, DNA templates were extracted from environmental samples from bathrooms in the residences of 29 patients with pulmonary M. avium disease. Of the 162 environmental samples examined, 143 (88%) showed identical results by both methods; 20 (12%) and 123 (76%) samples were positive and negative, respectively, for M. avium. Of the remaining 19 samples (12%), seven (5%) and 12 (7%) samples were positive by the LAMP and culture methods, respectively. All samples that contained over 20 colony forming units/primary isolation plate, as measured by the culture method, were also positive by the LAMP method. Our data demonstrate that the combination of the FTA elute card and LAMP can facilitate prompt detection of M. avium in the environment.
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Research Article|
November 25 2013
Direct detection of Mycobacterium avium in environmental water and scale samples by loop-mediated isothermal amplification
Yukiko Nishiuchi;
1Toneyama Institute for Tuberculosis Research, Osaka City University Medical School, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552, Japan
E-mail: [email protected]
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Aki Tamaru;
Aki Tamaru
2Department of Infectious Diseases, The Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan
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Yasuhiko Suzuki;
Yasuhiko Suzuki
3Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo, Hokkaido 001-0020, Japan
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Seigo Kitada;
Seigo Kitada
4National Hospital Organization Toneyama National Hospital, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552, Japan
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Ryoji Maekura;
Ryoji Maekura
4National Hospital Organization Toneyama National Hospital, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552, Japan
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Yoshitaka Tateishi;
Yoshitaka Tateishi
4National Hospital Organization Toneyama National Hospital, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552, Japan
5Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
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Mamiko Niki;
Mamiko Niki
5Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
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Hisashi Ogura;
Hisashi Ogura
6Department of Virology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
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Sohkichi Matsumoto
Sohkichi Matsumoto
5Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
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J Water Health (2014) 12 (2): 211–219.
Article history
Received:
January 04 2013
Accepted:
October 12 2013
Citation
Yukiko Nishiuchi, Aki Tamaru, Yasuhiko Suzuki, Seigo Kitada, Ryoji Maekura, Yoshitaka Tateishi, Mamiko Niki, Hisashi Ogura, Sohkichi Matsumoto; Direct detection of Mycobacterium avium in environmental water and scale samples by loop-mediated isothermal amplification. J Water Health 1 June 2014; 12 (2): 211–219. doi: https://doi.org/10.2166/wh.2013.007
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