Legionella pneumophila (L. pneumophila) is a Gram-negative bacterium, which can be found worldwide in aquatic environments. It tends to persist because it is often protected within biofilms or amoebae. L. pneumophila biofilms have a major impact on water systems, making the understanding of the bacterial physiological adaptation in biofilms a fundamental step towards their eradication. In this study, we report for the first time the influence of the biofilm mode of growth on the proteome of L. pneumophila. We compared the protein patterns of microorganisms grown as suspensions, cultured as colonies on agar plates or recovered with biofilms formed on stainless steel coupons. Statistical analyses of the protein expression data set confirmed the biofilm phenotype specificity which had been previously observed. It also identified dozens of proteins whose abundance was modified in biofilms. Proteins corresponding to virulence factors (macrophage infectivity potentiator protein, secreted proteases) were largely repressed in adherent cells. In contrast, a peptidoglycan-associated lipoprotein (Lpg2043) and a peroxynitrite reductase (Lpg2965) were accumulated by biofilm cells. Remarkably, hypothetical proteins, that appear to be unique to the Legionella genus (Lpg0563, Lpg1111 and Lpg1809), were over-expressed by sessile bacteria.
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Research Article|
January 08 2014
Proteomic regulation during Legionella pneumophila biofilm development: decrease of virulence factors and enhancement of response to oxidative stress
Arbia Khemiri
;
Arbia Khemiri
1
CNRS, UMR 6270, Laboratory Polymères, Biopolymères, Surfaces, Mont-Saint-Aignan, F-76820, France2
Normandy University, UR, France and PISSARO Proteomic Facility, IRIB, Mont-Saint-Aignan, F-76820, France
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Sandra Ahmed Lecheheb
;
Sandra Ahmed Lecheheb
1
CNRS, UMR 6270, Laboratory Polymères, Biopolymères, Surfaces, Mont-Saint-Aignan, F-76820, France2
Normandy University, UR, France and PISSARO Proteomic Facility, IRIB, Mont-Saint-Aignan, F-76820, France
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Philippe Chan Chi Song
;
Philippe Chan Chi Song
2
Normandy University, UR, France and PISSARO Proteomic Facility, IRIB, Mont-Saint-Aignan, F-76820, France3
Institute for Research and Innovation in Biomedecine (IRIB), University of Rouen, Mont-Saint-Aignan, 76821 Cedex, France
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Thierry Jouenne
;
Thierry Jouenne
1
CNRS, UMR 6270, Laboratory Polymères, Biopolymères, Surfaces, Mont-Saint-Aignan, F-76820, France2
Normandy University, UR, France and PISSARO Proteomic Facility, IRIB, Mont-Saint-Aignan, F-76820, France3
Institute for Research and Innovation in Biomedecine (IRIB), University of Rouen, Mont-Saint-Aignan, 76821 Cedex, France
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Pascal Cosette
1
CNRS, UMR 6270, Laboratory Polymères, Biopolymères, Surfaces, Mont-Saint-Aignan, F-76820, France2
Normandy University, UR, France and PISSARO Proteomic Facility, IRIB, Mont-Saint-Aignan, F-76820, France3
Institute for Research and Innovation in Biomedecine (IRIB), University of Rouen, Mont-Saint-Aignan, 76821 Cedex, France
E-mail: pascal.cosette@univ-rouen.fr
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J Water Health (2014) 12 (2): 242-253.
Article history
Received:
June 19 2013
Accepted:
October 23 2013
Citation
Arbia Khemiri, Sandra Ahmed Lecheheb, Philippe Chan Chi Song, Thierry Jouenne, Pascal Cosette; Proteomic regulation during Legionella pneumophila biofilm development: decrease of virulence factors and enhancement of response to oxidative stress. J Water Health 1 June 2014; 12 (2): 242–253. doi: https://doi.org/10.2166/wh.2014.103
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Arbia Khemiri, Sandra Ahmed Lecheheb, Philippe Chan Chi Song, Thierry Jouenne, Pascal Cosette; Proteomic regulation during Legionella pneumophila biofilm development: decrease of virulence factors and enhancement of response to oxidative stress. J Water Health 1 June 2014; 12 (2): 242–253. doi: https://doi.org/10.2166/wh.2014.103
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