Biosand filters (BSFs) are increasingly designed using smaller and/or lighter casing material in an effort to reduce logistical requirements and implementation costs. The increased portability of a smaller, lighter design presents a potential negative consequence: the ability to move the installed/operational filter by the homeowner and potentially disturb the system. This study investigated the effects of moving and agitation on filter performance, using mature BSFs which had been in use for over nine months prior to the move. Data were analyzed for four replicate filters of three different filter types: the traditional concrete BSF and two plastic bucket (5-gal and 2-gal, respectively; 5-gal bucket = 18.9-L bucket, 2-gal bucket = 7.6-L bucket) BSFs. Filters were moved approximately 1 km and monitored for hydraulic loading rates (HLRs) and Escherichia coli removal for 8 weeks following the move. Moving the filters resulted in reduced HLRs, likely due to sand compaction, but E. coli removal remained high (log10 removal ≥2.8 for all sizes) and increased significantly as compared to data collected prior to the move. The resulting operational implications of moving BSFs are discussed.