This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe2+ and pH values of 3, 4.5, and 6. The experiments were conducted according to a 32 experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe2+) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25–30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe2+) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.