Polyomavirus JC (JCPyV) is largely excreted by the human population through the urinary route and has been recognized as a potential viral marker for human waste contamination. This study aims to investigate the dissemination of JCPyV in waste water from a sewage treatment plant (STP) located in Rio de Janeiro, Brazil, and to describe the prevalence of JCPyV subtypes currently present in this population. Raw and treated sewage samples were collected bimonthly during one year, and examined for the presence of JCPyV using nested polymerase chain reaction (nPCR) and quantitative real time PCR (qPCR). JCPyV was detected by nPCR in 96% and 43% of raw and treated sewage samples, respectively. The concentration of JCPyV present in the samples ranged from 1.2 × 103 to 3.2 × 105 and 2.6 × 102 to 6.2 × 103 genome copies per 2 ml of concentrated raw and treated sewage sample, respectively. The strains were characterized and the obtained nucleotide sequences indicated that the detected JCPyV strains clustered with subtypes of East African, West African and European origin. To our knowledge, this is the first study describing the incidence and diversity of JCPyV strains in raw and treated sewage in Brazil.
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Research Article|
March 09 2010
Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil
Tulio Machado Fumian;
1Laboratório de Virologia Comparada e Ambiental, Pavilhão Hélio & Peggy Pereira, Avenida Brasil 4.365, Manguinhos, CEP 21040-360, Rio de Janeiro RJ, Brazil
Tel.: +55-21-2562-1851 Fax: +55-21-2562-1851; E-mail: [email protected]
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Flávia Ramos Guimarães;
Flávia Ramos Guimarães
2Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil
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Beatriz Jesus Pereira Vaz;
Beatriz Jesus Pereira Vaz
3Institute of Clinical Research Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil
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Marcus Tulius Teixeira da Silva;
Marcus Tulius Teixeira da Silva
3Institute of Clinical Research Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil
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Flávia Fontenelle Muylaert;
Flávia Fontenelle Muylaert
3Institute of Clinical Research Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil
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Silvia Bofill-Mas;
Silvia Bofill-Mas
4Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain
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Rosina Gironés;
Rosina Gironés
4Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain
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José Paulo G. Leite;
José Paulo G. Leite
2Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil
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Marize Pereira Miagostovich
Marize Pereira Miagostovich
2Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil
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J Water Health (2010) 8 (3): 438–445.
Article history
Received:
June 08 2009
Accepted:
November 19 2009
Citation
Tulio Machado Fumian, Flávia Ramos Guimarães, Beatriz Jesus Pereira Vaz, Marcus Tulius Teixeira da Silva, Flávia Fontenelle Muylaert, Silvia Bofill-Mas, Rosina Gironés, José Paulo G. Leite, Marize Pereira Miagostovich; Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil. J Water Health 1 September 2010; 8 (3): 438–445. doi: https://doi.org/10.2166/wh.2010.090
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