A comprehensive survey assessing the presence of Acanthamoeba was conducted on 50 samples from water sources in parks and public squares from 22 municipal districts of Tehran, Iran. The prevalence and genotypes of Acanthamoeba were determined by PCR and the PCR fragments of ribosomal RNA genes sequenced. Sixteen (32%) samples were positive for Acanthamoeba spp. Sequence analysis revealed that the positive isolates belonged to the T4 and T5 genotypes. Fourteen isolates (87.5%) were T4, and two (12.5%) were T5. Acanthamoeba may be a problematic organism for contact lens wearers and for immunocompromised individuals. In Iran, Acanthamoeba keratitis has increased in recent years, mainly due to poor hygiene in contact lens wearers. A thorough survey for the prevalence of this amoeba could have a significant role in prevention of disease. This is the first report of the T5 genotype from water in recreational areas of Tehran.
Skip Nav Destination
Article navigation
Research Article|
May 30 2011
Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran
M. Nazar;
M. Nazar
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
A. Haghighi;
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
E-mail: [email protected]
Search for other works by this author on:
M. Niyyati;
M. Niyyati
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
M. Eftekhar;
M. Eftekhar
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
F. Tahvildar-biderouni;
F. Tahvildar-biderouni
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
N. Taghipour;
N. Taghipour
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
A. Abadi;
A. Abadi
2Department of Community & Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
E. Nazemalhosseini Mojarad;
E. Nazemalhosseini Mojarad
3Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
A. Athari
A. Athari
1Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for other works by this author on:
J Water Health (2011) 9 (3): 603–608.
Article history
Received:
September 27 2010
Accepted:
March 10 2011
Citation
M. Nazar, A. Haghighi, M. Niyyati, M. Eftekhar, F. Tahvildar-biderouni, N. Taghipour, A. Abadi, E. Nazemalhosseini Mojarad, A. Athari; Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran. J Water Health 1 September 2011; 9 (3): 603–608. doi: https://doi.org/10.2166/wh.2011.152
Download citation file: