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The content of ammonia nitrogen in the simulated wastewater was low. The nitrogen, which is used by microorganisms, was insufficient. It was necessary to add the appropriate amount of ammonium chloride and potassium dihydrogen phosphate into the influent to supplement the nitrogen and phosphorus (C:N:P = 250:5:1) in this work. Glucose was added to the wastewater simultaneously as the co-substrate (1.032 g glucose COD is 1.0 g), forming the glucose and antibiotic solvent (MC, IPA, THF, DMF) mixed wastewater. The glucose concentration in the wastewater was gradually reduced, while the concentration of antibiotic solvent was increased in the experiment. Thus, the anaerobic sludge was domesticated, making it suitable for the degradation of antibiotic solvent. The influent eventually became a pure solvent of antibiotic wastewater. The dosages of glucose, buffer, nutrients and trace elements in the synthetic wastewater are shown in Table 2.

Table 2

Dosage of buffer, nutrient and trace element

ItemSolution concentration (g/L)Dosage (mg/gCOD)ItemSolution concentration (g/L)Dosage (mg/gCOD)
Glucose Solid Adjust according to the actual situation Sodium bicarbonate Solid Adjust according to the actual situation 
Nickel sulfate 440 5–12 Calcium chloride 260 10–25 
Ammonium chloride Solid 220–320 Ferric chloride 250 3–11 
Two potassium hydrogen phosphate 188 22–57 Manganese chloride 440 0.8–5.2 
Potassium dihydrogen phosphate 75 8–25 Zinc chloride 260 0.5–2.1 
Magnesium sulfate 320 32–84 Cobalt chloride 220 0.3–1.8 
Sodium citrate 170 18–86 Sodium metaborate 80 0.1–0.9 
Copper chloride 130 0.1–0.8 Ammonium molybdate 160 0.2–1.2 
ItemSolution concentration (g/L)Dosage (mg/gCOD)ItemSolution concentration (g/L)Dosage (mg/gCOD)
Glucose Solid Adjust according to the actual situation Sodium bicarbonate Solid Adjust according to the actual situation 
Nickel sulfate 440 5–12 Calcium chloride 260 10–25 
Ammonium chloride Solid 220–320 Ferric chloride 250 3–11 
Two potassium hydrogen phosphate 188 22–57 Manganese chloride 440 0.8–5.2 
Potassium dihydrogen phosphate 75 8–25 Zinc chloride 260 0.5–2.1 
Magnesium sulfate 320 32–84 Cobalt chloride 220 0.3–1.8 
Sodium citrate 170 18–86 Sodium metaborate 80 0.1–0.9 
Copper chloride 130 0.1–0.8 Ammonium molybdate 160 0.2–1.2 

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