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The richness (R) for all the DGGE band patterns was calculated by using the respective band data analyzed by the Quantity software. Numerical analysis of microbial community diversity and the dissimilarity among the samples is shown in Table 5. Based on the band patterns, the dissimilarity between seed sludge and the samples with and without AT addition ranged from 0.392 to 0.565. It could be concluded that the microbial communities in the DM samples G3 and G5 significantly changed, while the suspended sludge G2 and G4 with a lower dissimilarity of 0.342 provided the most similar among the samples. The results indicated that the AT addition contributed to the community abundance and structure with a significant change compared to the seed activated sludge. Moreover, compared to G4 and G5, the dissimilarity index (D) of G2 and G3 was almost the same as 0.4, indicating similar microbial diversity. In some instances (Sharp et al. 2011), the results proved that the microbial communities in DMBR may be similar. However, the higher diversity in the sDMBR with AT addition both for the DM and mixture liquid indicated that the microbial community was enriched after AT addition.

Table 5

Comparisons of the microbial diversity and dissimilarity of (G1) seed activated sludge, (G2) mixed liquid in AT added sDMBR, (G3) DM of AT added DMBR, (G4) mixed liquid in sDMBR, (G5) DM of sDMBR

SamplesRichnessMicrobial diversity (H)Dissimilarity to the seed sludge (D)Dissimilarity (D)Dissimilarity (D)Dissimilarity (D)
G1 28 2.458     
G2 37 2.970 0.433 0.444   
G3 38 3.230 0.392   
G4 30 2.987 0.492  0.410  
G5 31 2.470 0.516   
DM (G3,G5)      0.565 
Suspended (G2,G4)      0.342 
SamplesRichnessMicrobial diversity (H)Dissimilarity to the seed sludge (D)Dissimilarity (D)Dissimilarity (D)Dissimilarity (D)
G1 28 2.458     
G2 37 2.970 0.433 0.444   
G3 38 3.230 0.392   
G4 30 2.987 0.492  0.410  
G5 31 2.470 0.516   
DM (G3,G5)      0.565 
Suspended (G2,G4)      0.342 

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