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Analysis of variance (ANOVA) of the linear model is presented in Table 7. This explains the presence of variations in the net result caused by each factor that is relative to the total variation. According to a Pareto chart (Figure 2) the screened factors, in the order yeast extract, lactose and pH, were most effective for remazol golden yellow dye decolourisation.
Table 7

ANOVA for PBD

S. No.SourceDFSeq SSAdj SSAdj MSFP
Main effects 480.188 480.188 68.598 5.60 0.058* 
Lactose 108.661 108.661 108.661 8.87 0.041* 
Yeast extract 243.811 243.811 243.811 19.90 0.011* 
pH 100.283 100.283 100.283 8.18 0.046* 
Temperature 0.891 0.891 0.891 0.07 0.801 
Inoculum size 13.889 13.889 13.889 1.13 0.347 
Dye concentration 2.698 2.698 2.698 0.22 0.663 
Incubation period 9.955 9.955 9.955 0.81 0.418 
Residual error 49.015 49.015 12.254 – – 
 Total 11 529.204     
S. No.SourceDFSeq SSAdj SSAdj MSFP
Main effects 480.188 480.188 68.598 5.60 0.058* 
Lactose 108.661 108.661 108.661 8.87 0.041* 
Yeast extract 243.811 243.811 243.811 19.90 0.011* 
pH 100.283 100.283 100.283 8.18 0.046* 
Temperature 0.891 0.891 0.891 0.07 0.801 
Inoculum size 13.889 13.889 13.889 1.13 0.347 
Dye concentration 2.698 2.698 2.698 0.22 0.663 
Incubation period 9.955 9.955 9.955 0.81 0.418 
Residual error 49.015 49.015 12.254 – – 
 Total 11 529.204     

*Significant.

Figure 2

Effects of influencing factors on dye decolourisation in a Pareto chart.

Figure 2

Effects of influencing factors on dye decolourisation in a Pareto chart.

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