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The number of VGs detected in the TDE of enterococci did not differ significantly between the sites and the three sampling rounds (Table 3). A linear regression analysis applied (data not shown) found that the number of enterococci in the water samples did not correlate with the number of positive VGs observations (P = 0.6306).

Table 3

Number of VGs found in TDE of enterococci and the number of BPTs found in each sample

Sampling roundSiteNo. C-BPTsNo. S-BPTsNo. VGs in total enterococci DNADi
0.89 
 0.91 
 0.88 
 10 0.92 
 11 0.89 
 13 0.94 
 14 10 0.91 
 16 0.96 
 17 10 0.85 
Mean ± SD 7.8 ± 1.9a 3.1 ± 1.7 5.0 ± 0.87 0.91 ± 0.03 
0.92 
 10 0.94 
 0.70 
 0.86 
 0.46 
 12 0.56 
 15 0.94 
 16 0.77 
 17 10 0.88 
 18 0.80 
 19 0.83 
 20 0.99 
 21 0.77 
 22 0.96 
 23 0.73 
Mean ± SD 4.7 ± 3.2a,b 3.1 ± 1.8b 5.3 ± 0.49 0.81 ± 0.15 
10 0.91 
 0.96 
 0.96 
 0.83 
 0.89 
 0.86 
 11 0.88 
 13 0.96 
 14 10 0.96 
 16 0.83 
 17 10 0.85 
 18 0.80 
Mean ± SD 7.6 ± 2.2b 4.8 1.9b 5.5 ± 0.67 0.89 ± 0.06 
Sampling roundSiteNo. C-BPTsNo. S-BPTsNo. VGs in total enterococci DNADi
0.89 
 0.91 
 0.88 
 10 0.92 
 11 0.89 
 13 0.94 
 14 10 0.91 
 16 0.96 
 17 10 0.85 
Mean ± SD 7.8 ± 1.9a 3.1 ± 1.7 5.0 ± 0.87 0.91 ± 0.03 
0.92 
 10 0.94 
 0.70 
 0.86 
 0.46 
 12 0.56 
 15 0.94 
 16 0.77 
 17 10 0.88 
 18 0.80 
 19 0.83 
 20 0.99 
 21 0.77 
 22 0.96 
 23 0.73 
Mean ± SD 4.7 ± 3.2a,b 3.1 ± 1.8b 5.3 ± 0.49 0.81 ± 0.15 
10 0.91 
 0.96 
 0.96 
 0.83 
 0.89 
 0.86 
 11 0.88 
 13 0.96 
 14 10 0.96 
 16 0.83 
 17 10 0.85 
 18 0.80 
Mean ± SD 7.6 ± 2.2b 4.8 1.9b 5.5 ± 0.67 0.89 ± 0.06 

Di, Diversity index.

a,bIndicates a significant difference (P < 0.05).

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