Scenedesmus sp. (Accession No. JQ315576.1) is an indigenous strain isolated from freshwater (Table 1). In order to identify the strain based on 18S rRNA sequencing, a pure culture of microalgae on Bold Basal medium (BBM) was sent to Axil Scientific Pte Ltd (The Gemini, Singapore). The microalgae inoculum was prepared by sub-culturing in a BBM broth and incubated at room temperature (25 ± 2 °C, 12 h L:12 h D period) for 7 days as described by Bischoff & Bold (1963). The culture medium was centrifuged (6,000 rpm) to separate the microalgae cells; and the media residue was removed by washing using sterilized deionized water. The microalgae cells were then suspended in 10 mL of sterilized normal saline water. The concentrations of the cells were counted using a haemocytometer. Three inoculum concentrations were prepared with concentrations of 7, 8 and 9 log10 cells mL–1.
Accession number of Scenedesmus sp
| Description . | Max score . | Total score . | Query cover . | E value . | Ident . | Accession . |
|---|---|---|---|---|---|---|
| Scenedesmus sp. KMMCC 1534 18S ribosomal RNA gene, partial sequence | 3,528 | 3,528 | 70% | 0.0 | 98% | JQ315576.1 |
| Scenedesmus sp. KMMCC 1533 18S ribosomal RNA gene, partial sequence | 2,165 | 3,023 | 56% | 0.0 | 99% | JQ315577.1 |
| Scenedesmus sp. KMMCC 178 18S ribosomal RNA gene, partial sequence | 2,159 | 3,017 | 56% | 0.0 | 99% | JQ315566.1 |
| Scenedesmus sp. KMMCC 406 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315581.1 |
| Scenedesmus sp. KMMCC 1211 18S ribosomal RNA gene, partial sequence | 2,146 | 2,993 | 56% | 0.0 | 99% | JQ315570.1 |
| Scenedesmus sp. KMMCC 406 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315581.1 |
| Scenedesmus sp. KMMCC 872 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315579.1 |
| Scenedesmus sp. KMMCC 1258 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315574.1 |
| Scenedesmus sp. KMMCC 1211 18S ribosomal RNA gene, partial sequence | 2,146 | 2,993 | 56% | 0.0 | 99% | JQ315570.1 |
| Scenedesmus sp. UKM 9 18S ribosomal RNA gene, partial sequence | 2,141 | 3,065 | 57% | 0.0 | 99% | KU170547.1 |
| Scenedesmus sp. KMMCC 1297 18S ribosomal RNA gene, partial sequence | 2,135 | 2,993 | 56% | 0.0 | 99% | JQ315599.1 |
| Scenedesmus armatus isolate B 18S ribosomal RNA gene, partial sequence | 2,121 | 3,121 | 58% | 0.0 | 99% | KR082490.1 |
| Scenedesmus sp. Lake Las Vegas 18S ribosomal RNA gene, partial sequence | 2,111 | 3,049 | 57% | 0.0 | 99% | JX910112.1 |
| Scenedesmus abundans 18S ribosomal RNA gene, partial sequence | 2,065 | 2,868 | 53% | 0.0 | 99% | KT868823.1 |
| Description . | Max score . | Total score . | Query cover . | E value . | Ident . | Accession . |
|---|---|---|---|---|---|---|
| Scenedesmus sp. KMMCC 1534 18S ribosomal RNA gene, partial sequence | 3,528 | 3,528 | 70% | 0.0 | 98% | JQ315576.1 |
| Scenedesmus sp. KMMCC 1533 18S ribosomal RNA gene, partial sequence | 2,165 | 3,023 | 56% | 0.0 | 99% | JQ315577.1 |
| Scenedesmus sp. KMMCC 178 18S ribosomal RNA gene, partial sequence | 2,159 | 3,017 | 56% | 0.0 | 99% | JQ315566.1 |
| Scenedesmus sp. KMMCC 406 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315581.1 |
| Scenedesmus sp. KMMCC 1211 18S ribosomal RNA gene, partial sequence | 2,146 | 2,993 | 56% | 0.0 | 99% | JQ315570.1 |
| Scenedesmus sp. KMMCC 406 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315581.1 |
| Scenedesmus sp. KMMCC 872 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315579.1 |
| Scenedesmus sp. KMMCC 1258 18S ribosomal RNA gene, partial sequence | 2,154 | 3,017 | 56% | 0.0 | 99% | JQ315574.1 |
| Scenedesmus sp. KMMCC 1211 18S ribosomal RNA gene, partial sequence | 2,146 | 2,993 | 56% | 0.0 | 99% | JQ315570.1 |
| Scenedesmus sp. UKM 9 18S ribosomal RNA gene, partial sequence | 2,141 | 3,065 | 57% | 0.0 | 99% | KU170547.1 |
| Scenedesmus sp. KMMCC 1297 18S ribosomal RNA gene, partial sequence | 2,135 | 2,993 | 56% | 0.0 | 99% | JQ315599.1 |
| Scenedesmus armatus isolate B 18S ribosomal RNA gene, partial sequence | 2,121 | 3,121 | 58% | 0.0 | 99% | KR082490.1 |
| Scenedesmus sp. Lake Las Vegas 18S ribosomal RNA gene, partial sequence | 2,111 | 3,049 | 57% | 0.0 | 99% | JX910112.1 |
| Scenedesmus abundans 18S ribosomal RNA gene, partial sequence | 2,065 | 2,868 | 53% | 0.0 | 99% | KT868823.1 |