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In order to determine the effect of microbial wastewater quality on the contamination of lettuce, four different target concentrations of E. coli in wastewater were selected; 10, 102, 103 and 104E. coli most probable number (MPN)/100 mL. The wastewater samples (40 L) were collected at different points from the wastewater stabilisation ponds (WSP), Mt Barker wastewater treatment plant, South Australia. The concentration of E. coli in the various wastewaters used in the experiments and the collection points are shown in Table 1.

Table 1

The concentration of E. coli (MPN/100 mL) in wastewater samples from various sources

Target concentrationActual concentrationWastewater source (Mt Barkera)
10 6.3 The outlet of wastewater DAFbplant. 
102 75.9 Final pond in the WSP series 
103 1,299.7 The dilution of the first facultative pond: with the outlet water from the DAFb (10: 1) 
104 27,550 Wastewater from the first facultative pond 
Target concentrationActual concentrationWastewater source (Mt Barkera)
10 6.3 The outlet of wastewater DAFbplant. 
102 75.9 Final pond in the WSP series 
103 1,299.7 The dilution of the first facultative pond: with the outlet water from the DAFb (10: 1) 
104 27,550 Wastewater from the first facultative pond 

aMt Barker Community Wastewater Treatment Plant, South Australia.

bDAF: dissolved air flotation.

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