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Thirty-six samples in total were collected and analyzed by qPCR for viral presence. Viral genomes were detected in 30 samples (83.3%). The higher detection rate was observed for HAdV in 28 samples (77.8%); viral loads ranged from 1.35 × 102 to 4.08 × 104 genome equivalent copies per liter. EV was detected in only 8 samples (22.2%) in viral loads from 5.92 × 103 to 4.93 × 104 genome copies per liter. Thermotolerant coliform counting showed relatively low numbers in all samples with maximum of 240 MPN/100 mL. Results are summarized in Tables 2 and 3.

Table 2

Detection of AdV and EV (genomic copies/L) and thermotolerant coliforms (TC; MPN/100 mL) in water samples collected from the Ipanema beach, Porto Alegre, Brazil, during the period of November 2011 to April 2012

  Ipanema
Harvesting points 123
November AdV 2.53 × 102 ND 1.39 × 103 
EV ND ND ND 
CT 110 22 220 
December AdV 7.75 × 102 4.08 × 104 1.51 × 103 
EV ND ND ND 
CT 23 13 23 
January AdV ND ND 1.35 × 102 
EV ND ND ND 
CT 70 50 23 
February AdV 3.23 × 103 ND 1.62 × 103 
EV ND 1.21 × 104 ND 
CT 13 
March AdV 3.06 × 103 1.44 × 104 2.03 × 104 
EV ND ND ND 
CT 170 
April AdV 2.05 × 104 1.82 × 104 1.61 × 104 
EV ND ND ND 
CT 240 
  Ipanema
Harvesting points 123
November AdV 2.53 × 102 ND 1.39 × 103 
EV ND ND ND 
CT 110 22 220 
December AdV 7.75 × 102 4.08 × 104 1.51 × 103 
EV ND ND ND 
CT 23 13 23 
January AdV ND ND 1.35 × 102 
EV ND ND ND 
CT 70 50 23 
February AdV 3.23 × 103 ND 1.62 × 103 
EV ND 1.21 × 104 ND 
CT 13 
March AdV 3.06 × 103 1.44 × 104 2.03 × 104 
EV ND ND ND 
CT 170 
April AdV 2.05 × 104 1.82 × 104 1.61 × 104 
EV ND ND ND 
CT 240 

ND = not detected.

Table 3

Detection of AdV and EV (genomic copies/L) and thermotolerant coliforms (TC; MPN/100 mL) in water samples collected from the Lami beach, Porto Alegre, Brazil, during the period of November 2011 to April 2012

  Lami
Harvesting points123
November AdV ND 3.9 × 103 5.31 × 102 
EV ND 4.93 × 104 ND 
CT 50 33 13 
December AdV ND 9.04 × 103 3.65 × 102 
EV ND ND 5,92 × 103 
CT 
January AdV ND 8.72 × 103 3.75 × 103 
EV ND ND 1.35 × 104 
CT 23 13 
February AdV 5.46 × 103 4.44 × 103 2.16 × 103 
EV 1.81 × 104 1.03 × 104 ND 
CT 23 
March AdV 1.36 × 103 1.58 × 103 7.18 × 102 
EV 1.93 × 104 ND ND 
CT 
April AdV 6.94 × 103 3.54 × 103 ND 
EV ND ND 1.91 × 104 
CT 170 23 30 
  Lami
Harvesting points123
November AdV ND 3.9 × 103 5.31 × 102 
EV ND 4.93 × 104 ND 
CT 50 33 13 
December AdV ND 9.04 × 103 3.65 × 102 
EV ND ND 5,92 × 103 
CT 
January AdV ND 8.72 × 103 3.75 × 103 
EV ND ND 1.35 × 104 
CT 23 13 
February AdV 5.46 × 103 4.44 × 103 2.16 × 103 
EV 1.81 × 104 1.03 × 104 ND 
CT 23 
March AdV 1.36 × 103 1.58 × 103 7.18 × 102 
EV 1.93 × 104 ND ND 
CT 
April AdV 6.94 × 103 3.54 × 103 ND 
EV ND ND 1.91 × 104 
CT 170 23 30 

ND = not detected.

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