Thirty-six samples in total were collected and analyzed by qPCR for viral presence. Viral genomes were detected in 30 samples (83.3%). The higher detection rate was observed for HAdV in 28 samples (77.8%); viral loads ranged from 1.35 × 102 to 4.08 × 104 genome equivalent copies per liter. EV was detected in only 8 samples (22.2%) in viral loads from 5.92 × 103 to 4.93 × 104 genome copies per liter. Thermotolerant coliform counting showed relatively low numbers in all samples with maximum of 240 MPN/100 mL. Results are summarized in Tables 2 and 3.
Detection of AdV and EV (genomic copies/L) and thermotolerant coliforms (TC; MPN/100 mL) in water samples collected from the Ipanema beach, Porto Alegre, Brazil, during the period of November 2011 to April 2012
| . | . | Ipanema . | ||
|---|---|---|---|---|
| Harvesting points . | . | 1 . | 2 . | 3 . |
| November | AdV | 2.53 × 102 | ND | 1.39 × 103 |
| EV | ND | ND | ND | |
| CT | 110 | 22 | 220 | |
| December | AdV | 7.75 × 102 | 4.08 × 104 | 1.51 × 103 |
| EV | ND | ND | ND | |
| CT | 23 | 13 | 23 | |
| January | AdV | ND | ND | 1.35 × 102 |
| EV | ND | ND | ND | |
| CT | 70 | 50 | 23 | |
| February | AdV | 3.23 × 103 | ND | 1.62 × 103 |
| EV | ND | 1.21 × 104 | ND | |
| CT | 13 | 2 | 4 | |
| March | AdV | 3.06 × 103 | 1.44 × 104 | 2.03 × 104 |
| EV | ND | ND | ND | |
| CT | 170 | 2 | 2 | |
| April | AdV | 2.05 × 104 | 1.82 × 104 | 1.61 × 104 |
| EV | ND | ND | ND | |
| CT | 8 | 4 | 240 | |
| . | . | Ipanema . | ||
|---|---|---|---|---|
| Harvesting points . | . | 1 . | 2 . | 3 . |
| November | AdV | 2.53 × 102 | ND | 1.39 × 103 |
| EV | ND | ND | ND | |
| CT | 110 | 22 | 220 | |
| December | AdV | 7.75 × 102 | 4.08 × 104 | 1.51 × 103 |
| EV | ND | ND | ND | |
| CT | 23 | 13 | 23 | |
| January | AdV | ND | ND | 1.35 × 102 |
| EV | ND | ND | ND | |
| CT | 70 | 50 | 23 | |
| February | AdV | 3.23 × 103 | ND | 1.62 × 103 |
| EV | ND | 1.21 × 104 | ND | |
| CT | 13 | 2 | 4 | |
| March | AdV | 3.06 × 103 | 1.44 × 104 | 2.03 × 104 |
| EV | ND | ND | ND | |
| CT | 170 | 2 | 2 | |
| April | AdV | 2.05 × 104 | 1.82 × 104 | 1.61 × 104 |
| EV | ND | ND | ND | |
| CT | 8 | 4 | 240 | |
ND = not detected.
Detection of AdV and EV (genomic copies/L) and thermotolerant coliforms (TC; MPN/100 mL) in water samples collected from the Lami beach, Porto Alegre, Brazil, during the period of November 2011 to April 2012
| . | . | Lami . | ||
|---|---|---|---|---|
| Harvesting points . | . | 1 . | 2 . | 3 . |
| November | AdV | ND | 3.9 × 103 | 5.31 × 102 |
| EV | ND | 4.93 × 104 | ND | |
| CT | 50 | 33 | 13 | |
| December | AdV | ND | 9.04 × 103 | 3.65 × 102 |
| EV | ND | ND | 5,92 × 103 | |
| CT | 8 | 2 | 2 | |
| January | AdV | ND | 8.72 × 103 | 3.75 × 103 |
| EV | ND | ND | 1.35 × 104 | |
| CT | 8 | 23 | 13 | |
| February | AdV | 5.46 × 103 | 4.44 × 103 | 2.16 × 103 |
| EV | 1.81 × 104 | 1.03 × 104 | ND | |
| CT | 2 | 23 | 2 | |
| March | AdV | 1.36 × 103 | 1.58 × 103 | 7.18 × 102 |
| EV | 1.93 × 104 | ND | ND | |
| CT | 2 | 2 | 2 | |
| April | AdV | 6.94 × 103 | 3.54 × 103 | ND |
| EV | ND | ND | 1.91 × 104 | |
| CT | 170 | 23 | 30 | |
| . | . | Lami . | ||
|---|---|---|---|---|
| Harvesting points . | . | 1 . | 2 . | 3 . |
| November | AdV | ND | 3.9 × 103 | 5.31 × 102 |
| EV | ND | 4.93 × 104 | ND | |
| CT | 50 | 33 | 13 | |
| December | AdV | ND | 9.04 × 103 | 3.65 × 102 |
| EV | ND | ND | 5,92 × 103 | |
| CT | 8 | 2 | 2 | |
| January | AdV | ND | 8.72 × 103 | 3.75 × 103 |
| EV | ND | ND | 1.35 × 104 | |
| CT | 8 | 23 | 13 | |
| February | AdV | 5.46 × 103 | 4.44 × 103 | 2.16 × 103 |
| EV | 1.81 × 104 | 1.03 × 104 | ND | |
| CT | 2 | 23 | 2 | |
| March | AdV | 1.36 × 103 | 1.58 × 103 | 7.18 × 102 |
| EV | 1.93 × 104 | ND | ND | |
| CT | 2 | 2 | 2 | |
| April | AdV | 6.94 × 103 | 3.54 × 103 | ND |
| EV | ND | ND | 1.91 × 104 | |
| CT | 170 | 23 | 30 | |
ND = not detected.