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Surveys of fecal indicator bacteria and P. aeruginosa in the spray water were conducted from early September to early October (for the first survey) and from late October to late November (for the second survey). The detection rates of fecal indicator bacteria and P. aeruginosa were nearly identical in both surveys (Table 3). Coliform bacteria were detected in the spray water collected from seven toilet seats (5.5%), E. coli was detected in the water from three toilet seats (2.4%), enterococci were detected in the water from four toilet seats (3.1%), and either E. coli or enterococci were detected in the water from six toilet seats (4.7%). When tap water was analyzed as a negative control, no fecal indicator bacteria were detected, as expected.

Table 3

Detection of fecal indicator bacteria and P. aeruginosa

  Coliforms
E. coli
Enterococcus
E. coli or enterococcus
P. aeruginosa
SurveynPositive numberDetection ratio (%)Positive numberDetection ratio (%)Positive numberDetection ratio (%)Positive numberDetection ratio (%)Positive numberDetection ratio (%)
1st Total 127 5.5 2.4 3.1 4.7 1.6 
Male 43 14.0 7.0 4.7 3.1 2.3 
Female 71 1.4 0.0 2.8 1.6 1.4 
Barrier-Free 13 0.0 0.0 0.0 0.0 0.0 
2nd Total 127 4.7 4.7 2.4 4.7 1.6 
Male 43 11.6 11.6 2.3 3.9 2.3 
Female 71 1.4 1.4 2.8 0.8 1.4 
Barrier-Free 13 0.0 0.0 0.0 0.0 0.0 
1st + 2nd Total 254 13 5.1 3.5 2.8 15 5.9 1.6 
Male 86 11 12.8 9.3 3.5 11 4.3 2.3 
Female 142 1.4 0.7 2.8 1.6 1.4 
Barrier-Free 26 0.0 0.0 0.0 0.0 0.0 
  Coliforms
E. coli
Enterococcus
E. coli or enterococcus
P. aeruginosa
SurveynPositive numberDetection ratio (%)Positive numberDetection ratio (%)Positive numberDetection ratio (%)Positive numberDetection ratio (%)Positive numberDetection ratio (%)
1st Total 127 5.5 2.4 3.1 4.7 1.6 
Male 43 14.0 7.0 4.7 3.1 2.3 
Female 71 1.4 0.0 2.8 1.6 1.4 
Barrier-Free 13 0.0 0.0 0.0 0.0 0.0 
2nd Total 127 4.7 4.7 2.4 4.7 1.6 
Male 43 11.6 11.6 2.3 3.9 2.3 
Female 71 1.4 1.4 2.8 0.8 1.4 
Barrier-Free 13 0.0 0.0 0.0 0.0 0.0 
1st + 2nd Total 254 13 5.1 3.5 2.8 15 5.9 1.6 
Male 86 11 12.8 9.3 3.5 11 4.3 2.3 
Female 142 1.4 0.7 2.8 1.6 1.4 
Barrier-Free 26 0.0 0.0 0.0 0.0 0.0 

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