A Chi-squared test of the fecal indicator bacteria detection rates in male and female restrooms showed a tendency for coliform bacteria to be detected more often in spray water from toilet seats in male restrooms (P < 0.05) than in female restrooms. Most fecal indicator bacteria were detected at a concentration of 1 MPN/mL or less (Table 4), and fecal indicator bacteria (coliform bacteria, E. coli, enterococci) appeared in only one toilet seat in both surveys (Table 4, No. 6). This indicates that feces stuck to the nozzle that discharges spray water is able to mix with the spray water when it comes out, although in very small amounts, leading to the detection of fecal indicator bacteria in spray water.