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Spray waters 1–5 were collected from the research building and spray water 6 was collected from a location about 6 km from the university campus. However, the tap water supplying all of the toilets was from the same water source and the same water-purification plant. The band patterns in Figure 7 show two systems (band a, bands b and c) in almost the same location in spray waters 1–6; these are thought to represent bacteria from the shared water source. Note that band groups are evident below bands a1 to a6 in Figure 7, but these bands could not be confirmed or cut out. Sequence analysis was performed on the cutout bands shown in Figure 7, and the sequences that were obtained were identified by a Basic Local Alignment Search Tool (BLAST) search of the DNA Data Bank of Japan (Table 5).

Table 5

Identification of denaturing gradient gel electrophoresis bands

BandAccession numberMost closely related organismsPartial sequence identitiesGap
a1, a2 NR 024783.1 Arthrobacter russicus strain A1-3 1 186/188 (99%) 2/188 (1%) 
a4 NR 024783.1 Arthrobacter russicus strain A1-3 151/152 (99%) 1/152 (1%) 
a5 NR 024783.1 Arthrobacter russicus strain A1-3 184/188 (98%) 2/188 (1%) 
a6 NR 024783.1 Arthrobacter russicus strain A1-3 185/188 (98%) 2/188 (1%) 
b2, b5 NR 044320.1 Sphingomonadaceae bacterium E4A9 strain 169/169 (100%)  
NR 040827.1 Novosphingobium subterraneum strain IFO 16086 169/169 (100%)  
NR 025838.1 Novosphingobium capsulatum strain GIFU 11526 169/169 (100%)  
c1, c3, c4_2, c5, c6_2 NR 044187.1 Sphingomonas insulae strain DS-28 169/169 (100%)  
NR 043612.1 Sphingomonas dokdonensis strain DS-4 169/169 (100%)  
NR 024631.1 Sphingopyxis chilensis strain S37 169/169 (100%)  
d2 NR 042358.1 Mesorhizobium thiogangeticum strain 169/169 (100%)  
NR 028901.1 Ochrobactrum grignonense strain OgA9a 169/169 (100%)  
e2 NR 044095.1 Filomicrobium insigne strain SLG5B-19 165/169 (98%) 0/169 (0%) 
f2, f5 NR 036877.1 Craurococcus roseus strain NS130 168/169 (99%) 0/169 (0%) 
BandAccession numberMost closely related organismsPartial sequence identitiesGap
a1, a2 NR 024783.1 Arthrobacter russicus strain A1-3 1 186/188 (99%) 2/188 (1%) 
a4 NR 024783.1 Arthrobacter russicus strain A1-3 151/152 (99%) 1/152 (1%) 
a5 NR 024783.1 Arthrobacter russicus strain A1-3 184/188 (98%) 2/188 (1%) 
a6 NR 024783.1 Arthrobacter russicus strain A1-3 185/188 (98%) 2/188 (1%) 
b2, b5 NR 044320.1 Sphingomonadaceae bacterium E4A9 strain 169/169 (100%)  
NR 040827.1 Novosphingobium subterraneum strain IFO 16086 169/169 (100%)  
NR 025838.1 Novosphingobium capsulatum strain GIFU 11526 169/169 (100%)  
c1, c3, c4_2, c5, c6_2 NR 044187.1 Sphingomonas insulae strain DS-28 169/169 (100%)  
NR 043612.1 Sphingomonas dokdonensis strain DS-4 169/169 (100%)  
NR 024631.1 Sphingopyxis chilensis strain S37 169/169 (100%)  
d2 NR 042358.1 Mesorhizobium thiogangeticum strain 169/169 (100%)  
NR 028901.1 Ochrobactrum grignonense strain OgA9a 169/169 (100%)  
e2 NR 044095.1 Filomicrobium insigne strain SLG5B-19 165/169 (98%) 0/169 (0%) 
f2, f5 NR 036877.1 Craurococcus roseus strain NS130 168/169 (99%) 0/169 (0%) 

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