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The high-performance liquid chromatograph (HPLC) grade TC with the purity of ≥88% was purchased from Sigma-Aldrich Chemical Co. (USA) and used without further purification. The chemical structure and some main characteristics of the TC are presented in Table 1. Methanol and acetonitrile (both HPLC grade), and oxalic acid dehydrate were purchased from Merck Company, Germany. Deionized water was used throughout the experiments to prepare working solutions. All other chemicals used were analytical reagent grade and used without further purification.

Table 1

Chemical structure and main characteristics of TC antibiotic

NameChemical formulaMolecular weightMolecular structurepKa1pKa2pKa3
TC C22H24N2O8·xH2444.43  3.3 7.68 9.68 
NameChemical formulaMolecular weightMolecular structurepKa1pKa2pKa3
TC C22H24N2O8·xH2444.43  3.3 7.68 9.68 

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