Comparison of the molecular source identifier copy numbers generated by RNA-based RT-qPCR and DNA-based qPCR methods using Enterococcus spp. (Entero1), E. coli (EC23S857), Bacteroidetes spp. (GenBac3), human-specific Bacteroidetes (HF183), and gull-specific Catellicoccus marimammalium (Gull4) assays
| Assay . | RNA-based signal higher . | Equal or non-detect . | DNA-based signal higher . |
|---|---|---|---|
| Entero1 | 6/19 (32%) | 12/19 (63%) | 1/19 (5%) |
| EC23S857 | 10/19 (53%) | 0/19 (0%) | 9/19 (47%) |
| GenBac3 | 11/19 (58%) | 0/19 (0%) | 8/19 (42%) |
| HF183 | 5/19 (26%) | 12/19 (63%) | 2/19 (11%) |
| Gull4 | 9/19 (47%) | 5/19 (26%) | 5/19 (26%) |
| Assay . | RNA-based signal higher . | Equal or non-detect . | DNA-based signal higher . |
|---|---|---|---|
| Entero1 | 6/19 (32%) | 12/19 (63%) | 1/19 (5%) |
| EC23S857 | 10/19 (53%) | 0/19 (0%) | 9/19 (47%) |
| GenBac3 | 11/19 (58%) | 0/19 (0%) | 8/19 (42%) |
| HF183 | 5/19 (26%) | 12/19 (63%) | 2/19 (11%) |
| Gull4 | 9/19 (47%) | 5/19 (26%) | 5/19 (26%) |