Skip to Main Content
Table 1

Primer sequences and length of amplification products

Target DNA fragmentPrimerOligonucleotide sequence (5′ − 3′)Length of amplification productReferences
16S rDNA region V3 for RT-qPCR 338F CCTACGGGAGGCAGCAG 181 bp An et al. (2012); Zhang et al. (2016)  
518R ATTACCGCGGCTGCTGG 
Partial NirS gene for RT-qPCR NirS-F CACGGYGTBCTGCGCAAGGGCGC 702 bp 
NirS-F-R CGCCACGCGCGGYTCSGGGTGGTA Scala & Kerkhof (1998); Bai et al. (2008)  
Partial NosZ gene for RT-qPCR NirS-F-F CGYTGTTCMTCGACAGCCAG 707 bp 
NirS-F-R CATGTGCAGNGCRTGGCAGAA 
Target DNA fragmentPrimerOligonucleotide sequence (5′ − 3′)Length of amplification productReferences
16S rDNA region V3 for RT-qPCR 338F CCTACGGGAGGCAGCAG 181 bp An et al. (2012); Zhang et al. (2016)  
518R ATTACCGCGGCTGCTGG 
Partial NirS gene for RT-qPCR NirS-F CACGGYGTBCTGCGCAAGGGCGC 702 bp 
NirS-F-R CGCCACGCGCGGYTCSGGGTGGTA Scala & Kerkhof (1998); Bai et al. (2008)  
Partial NosZ gene for RT-qPCR NirS-F-F CGYTGTTCMTCGACAGCCAG 707 bp 
NirS-F-R CATGTGCAGNGCRTGGCAGAA 
Close Modal

or Create an Account

Close Modal
Close Modal