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Table 1

Primers used for the identification of E. coli pathogenic strains (Omar & Barnard 2014)

PathogenPrimerSequenceSize (bp)Reference
E. coli Mdh (F)
Mdh (R) 
GGT ATG GAT CGT TCC GAC CT
GGCAGA ATG GTA ACA CCA GAG T 
300 Tarr et al. (2002)  
EIEC ial (F)
ial (R) 
GGT ATG ATG ATG ATG AGT GGC
GGA GGC CAA CAA TTA TTT CC 
630 López Saucedo et al. (2003)  
EHEC/Atypical EPEC eaeA (F)
eaeA (R) 
CTG AAC GGC GAT TAC GCG AA
GAC GAT ACG ATC CAG 
917 Aranda et al. (2004)  
Typical EPEC bfpA (F)
bfpM (R) 
AAT GGT GCT TGC GCT TGC TGC
TAT TAA CAC CGT AGC CTT TCG CTG AAG TAC CT 
410 Aranda et al. (2004)  
EAEC stxl (F)
stxl (R)
stx2 (F)
stx2 (R) 
ACA CTG GAT GAT CTC AGT GG
CTG AAT CCC CCT CCA TTA TG
CCA TGA CAA CGG ACA GCA GTT
CCT GTC AAC TGA GCA CTT TG 
614

779 
Moses et al. (2006)  
ETEC It (F)
It (R)
ST (F)
ST (R) 
GGC GAC AGA TTA TAC CGT GC
CGG TCT CTA TAT TCC CTG TT
TTT CCC CTC TTT TAG TCA GTC AAC TG
GGC AGG ATT ACA ACA AAG TTC ACA 
360

160 
Pass et al. (2000)  
PathogenPrimerSequenceSize (bp)Reference
E. coli Mdh (F)
Mdh (R) 
GGT ATG GAT CGT TCC GAC CT
GGCAGA ATG GTA ACA CCA GAG T 
300 Tarr et al. (2002)  
EIEC ial (F)
ial (R) 
GGT ATG ATG ATG ATG AGT GGC
GGA GGC CAA CAA TTA TTT CC 
630 López Saucedo et al. (2003)  
EHEC/Atypical EPEC eaeA (F)
eaeA (R) 
CTG AAC GGC GAT TAC GCG AA
GAC GAT ACG ATC CAG 
917 Aranda et al. (2004)  
Typical EPEC bfpA (F)
bfpM (R) 
AAT GGT GCT TGC GCT TGC TGC
TAT TAA CAC CGT AGC CTT TCG CTG AAG TAC CT 
410 Aranda et al. (2004)  
EAEC stxl (F)
stxl (R)
stx2 (F)
stx2 (R) 
ACA CTG GAT GAT CTC AGT GG
CTG AAT CCC CCT CCA TTA TG
CCA TGA CAA CGG ACA GCA GTT
CCT GTC AAC TGA GCA CTT TG 
614

779 
Moses et al. (2006)  
ETEC It (F)
It (R)
ST (F)
ST (R) 
GGC GAC AGA TTA TAC CGT GC
CGG TCT CTA TAT TCC CTG TT
TTT CCC CTC TTT TAG TCA GTC AAC TG
GGC AGG ATT ACA ACA AAG TTC ACA 
360

160 
Pass et al. (2000)  

F = forward.

R = reverse.

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