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Table 2

Assessment of consumables cost per sample and process duration of four eDNA extraction methods

Extraction methodsTime for sample preparationTime DNA extraction/plate (96 samples)Cost ($)/sampleSafeHigh throughput
PCI – 6 h 2.5 No a 
Bead-Robotic 3 h 20 min Yes Yes 
Bead-Manual 3 h 2 h Yes – 
Membrane-filtration 3 h 20 min No Yes 
Extraction methodsTime for sample preparationTime DNA extraction/plate (96 samples)Cost ($)/sampleSafeHigh throughput
PCI – 6 h 2.5 No a 
Bead-Robotic 3 h 20 min Yes Yes 
Bead-Manual 3 h 2 h Yes – 
Membrane-filtration 3 h 20 min No Yes 

aHard to be high-throughput.

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