In vitro growth of Acanthamoeba isolated from recreational hot springs at different temperatures and osmolarities
| Sample code . | Sampling site . | Genotype . | Thermotolerance assay . | Osmotolerance assay . | ||
|---|---|---|---|---|---|---|
| At 37 °C . | At 42 °C . | 0.5 M mannitol . | 1 M mannitol . | |||
| SA1-SLG1 | Selayang | T4 | +++ | ++ | ++ | − |
| SA2-SLG2 | Selayang | T4 | +++ | ++ | ++ | − |
| SA3-SLG3 | Selayang | T4 | +++ | ++ | + | − |
| SD1-SLG9 | Selayang | T4 | +++ | ++ | + | ++ |
| SD2-SLG10 | Selayang | T4 | +++ | ++ | ++ | − |
| UTA1-HT11 | Hulu Tamu | T15 | +++ | + | − | − |
| UTA2-HT12 | Hulu Tamu | T4 | +++ | + | ++ | − |
| UTA3-HT13 | Hulu Tamu | T4 | +++ | + | + | − |
| UTA4-HT14 | Hulu Tamu | T4 | +++ | + | ++ | + |
| UTA5-HT15 | Hulu Tamu | T4 | +++ | − | ++ | − |
| UTB1-HT16 | Hulu Tamu | T15 | +++ | − | ++ | − |
| UTB2-HT17 | Hulu Tamu | T15 | +++ | − | − | − |
| UTB3-HT18 | Hulu Tamu | T15 | +++ | − | ++ | − |
| UTB4-HT19 | Hulu Tamu | T15 | +++ | − | + | − |
| UTC1-HT20 | Hulu Tamu | T15 | +++ | + | + | + |
| BTGA3-BTG23 | Bentong | T15 | +++ | + | − | − |
| BTGA4-BTG24 | Bentong | T4 | +++ | + | ++ | − |
| BTGA5-BTG25 | Bentong | T4 | +++ | + | + | − |
| BTGB1-BTG26 | Bentong | T15 | +++ | ++ | + | − |
| BTGB3-BTG28 | Bentong | T11 | +++ | ++ | + | − |
| BTGB4-BTG29 | Bentong | T4 | +++ | ++ | + | − |
| BTGB5-BTG30 | Bentong | T17 | +++ | ++ | ++ | − |
| SKA1-SK31 | Sungai Klah | T4 | +++ | − | ++ | − |
| SKA3-SK33 | Sungai Klah | T3 | +++ | ++ | ++ | − |
| SKA4-SK34 | Sungai Klah | T4 | +++ | − | ++ | − |
| SKA5-SK35 | Sungai Klah | T3 | +++ | + | + | + |
| SKB1-SK36 | Sungai Klah | T4 | +++ | ++ | ++ | − |
| SKB2-SK37 | Sungai Klah | T5 | +++ | + | ++ | − |
| SKC2-SK40 | Sungai Klah | T4 | +++ | + | ++ | − |
| GA2-GDK42 | Gadek | T15 | +++ | ++ | ++ | − |
| GB1-GDK43 | Gadek | T4 | +++ | ++ | ++ | − |
| GB2-GDK44 | Gadek | T4 | +++ | ++ | ++ | + |
| GB3-GDK45 | Gadek | T4 | +++ | ++ | ++ | − |
| GB4-GDK46 | Gadek | T4 | +++ | − | ++ | − |
| GC1-GDK47 | Gadek | T4 | +++ | ++ | ++ | − |
| GC2-GDK48 | Gadek | T4 | +++ | ++ | ++ | − |
| GC3-GDK49 | Gadek | T4 | +++ | + | ++ | + |
| GC4-GDK50 | Gadek | T4 | +++ | ++ | ++ | − |
| Reference strain Acanthamoeba castellanii ATCC 50492 | T4 | +++ | ++ | +++ | ++ | |
| Sample code . | Sampling site . | Genotype . | Thermotolerance assay . | Osmotolerance assay . | ||
|---|---|---|---|---|---|---|
| At 37 °C . | At 42 °C . | 0.5 M mannitol . | 1 M mannitol . | |||
| SA1-SLG1 | Selayang | T4 | +++ | ++ | ++ | − |
| SA2-SLG2 | Selayang | T4 | +++ | ++ | ++ | − |
| SA3-SLG3 | Selayang | T4 | +++ | ++ | + | − |
| SD1-SLG9 | Selayang | T4 | +++ | ++ | + | ++ |
| SD2-SLG10 | Selayang | T4 | +++ | ++ | ++ | − |
| UTA1-HT11 | Hulu Tamu | T15 | +++ | + | − | − |
| UTA2-HT12 | Hulu Tamu | T4 | +++ | + | ++ | − |
| UTA3-HT13 | Hulu Tamu | T4 | +++ | + | + | − |
| UTA4-HT14 | Hulu Tamu | T4 | +++ | + | ++ | + |
| UTA5-HT15 | Hulu Tamu | T4 | +++ | − | ++ | − |
| UTB1-HT16 | Hulu Tamu | T15 | +++ | − | ++ | − |
| UTB2-HT17 | Hulu Tamu | T15 | +++ | − | − | − |
| UTB3-HT18 | Hulu Tamu | T15 | +++ | − | ++ | − |
| UTB4-HT19 | Hulu Tamu | T15 | +++ | − | + | − |
| UTC1-HT20 | Hulu Tamu | T15 | +++ | + | + | + |
| BTGA3-BTG23 | Bentong | T15 | +++ | + | − | − |
| BTGA4-BTG24 | Bentong | T4 | +++ | + | ++ | − |
| BTGA5-BTG25 | Bentong | T4 | +++ | + | + | − |
| BTGB1-BTG26 | Bentong | T15 | +++ | ++ | + | − |
| BTGB3-BTG28 | Bentong | T11 | +++ | ++ | + | − |
| BTGB4-BTG29 | Bentong | T4 | +++ | ++ | + | − |
| BTGB5-BTG30 | Bentong | T17 | +++ | ++ | ++ | − |
| SKA1-SK31 | Sungai Klah | T4 | +++ | − | ++ | − |
| SKA3-SK33 | Sungai Klah | T3 | +++ | ++ | ++ | − |
| SKA4-SK34 | Sungai Klah | T4 | +++ | − | ++ | − |
| SKA5-SK35 | Sungai Klah | T3 | +++ | + | + | + |
| SKB1-SK36 | Sungai Klah | T4 | +++ | ++ | ++ | − |
| SKB2-SK37 | Sungai Klah | T5 | +++ | + | ++ | − |
| SKC2-SK40 | Sungai Klah | T4 | +++ | + | ++ | − |
| GA2-GDK42 | Gadek | T15 | +++ | ++ | ++ | − |
| GB1-GDK43 | Gadek | T4 | +++ | ++ | ++ | − |
| GB2-GDK44 | Gadek | T4 | +++ | ++ | ++ | + |
| GB3-GDK45 | Gadek | T4 | +++ | ++ | ++ | − |
| GB4-GDK46 | Gadek | T4 | +++ | − | ++ | − |
| GC1-GDK47 | Gadek | T4 | +++ | ++ | ++ | − |
| GC2-GDK48 | Gadek | T4 | +++ | ++ | ++ | − |
| GC3-GDK49 | Gadek | T4 | +++ | + | ++ | + |
| GC4-GDK50 | Gadek | T4 | +++ | ++ | ++ | − |
| Reference strain Acanthamoeba castellanii ATCC 50492 | T4 | +++ | ++ | +++ | ++ | |
Scores of −, +, ++ and +++ indicated for 0, 1–15, 16–30 and >30 cysts and/or trophozoites, respectively.