Skip to Main Content
Table 1

Oligonucleotide sequences (primers/probes) for qPCR optimisation

TargetPrimers/probes (5′–3′)Product size (bp)GeneSource
Ascaris spp. Forward-GTAATAGCAGTCGGCGGTTTCTT 88 ITS1 Llewellyn et al. (2016)  
  Reverse-GCCCAACATGCCACCTATTC    
  Probe
ROX-TTGGCGGACAATTGCATGCGAT-IBRQ 
   
EH Virus F-GATGACACTAGCGACTTCGA 81 gB Llewellyn et al. (2016)  
  R-CAGGGCAGAAACCATAGACA    
  Probe
CY5/FAM-TTTCGCGTGCCTCCTCCAG-IBRQ/IBFQ 
   
TargetPrimers/probes (5′–3′)Product size (bp)GeneSource
Ascaris spp. Forward-GTAATAGCAGTCGGCGGTTTCTT 88 ITS1 Llewellyn et al. (2016)  
  Reverse-GCCCAACATGCCACCTATTC    
  Probe
ROX-TTGGCGGACAATTGCATGCGAT-IBRQ 
   
EH Virus F-GATGACACTAGCGACTTCGA 81 gB Llewellyn et al. (2016)  
  R-CAGGGCAGAAACCATAGACA    
  Probe
CY5/FAM-TTTCGCGTGCCTCCTCCAG-IBRQ/IBFQ 
   
Close Modal

or Create an Account

Close Modal
Close Modal