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Table 1

Primers used in this study

FLA^a	Primer sequence	References
N. fowleri^b	F5′-GTGAAAACCTTTTTTCCATTTACA-3′ R5′-AAATAAAAGATTGACCATTTGAAA-3′	Pelandakis et al. (2000); Latifi et al. (2017)
Vahlkampfiids	ITS1 F5′-GAACCTGCGTAGGGATCATTT-3′ ITS2 R 5'TTTCTTTTCCTCCCCTTATTA-3′	Pelandakis et al. (2000); Javanmard et al. (2017)
Acanthamoeba spp.	JDP15′-GGCCCAGATCGTTTACCGTGAA-3′ JDP2 5′-TCTCACAAGCTGCTAGGGAGTCA-3′	Mahmoudi et al. (2015b)
Vermamoeba	NA1F 5′-TTA CGA GGT CAG GAC ACTGT-3′ NA2R 5′-GAC CAT CCG GAG TTC TCG-3′	Javanmard et al. (2017)

FLA^a	Primer sequence	References
N. fowleri^b	F5′-GTGAAAACCTTTTTTCCATTTACA-3′ R5′-AAATAAAAGATTGACCATTTGAAA-3′	Pelandakis et al. (2000); Latifi et al. (2017)
Vahlkampfiids	ITS1 F5′-GAACCTGCGTAGGGATCATTT-3′ ITS2 R 5'TTTCTTTTCCTCCCCTTATTA-3′	Pelandakis et al. (2000); Javanmard et al. (2017)
Acanthamoeba spp.	JDP15′-GGCCCAGATCGTTTACCGTGAA-3′ JDP2 5′-TCTCACAAGCTGCTAGGGAGTCA-3′	Mahmoudi et al. (2015b)
Vermamoeba	NA1F 5′-TTA CGA GGT CAG GAC ACTGT-3′ NA2R 5′-GAC CAT CCG GAG TTC TCG-3′	Javanmard et al. (2017)

^aFree living amoebae.

^bNaegleria fawleri.