Primers and probes used in this study
| Assay . | Primer and probe sequences (5′–3′) . | Reference . |
|---|---|---|
| Human mtDNA | Forward: CAGCAGCCATTCAAGCAATGC Reverse: GGTGGAGACCTAATTGGGCTGATTAG Probe: TATCGGCGATATCGGTTTCATCCTCG | Caldwell et al. (2007) |
| Human-associated Bacteroidales (HF183) | HF183-1: ATCATGAGTTCACATGTCCG BthetR1: CGTAGGAGTTTGGACCGTGT BthetP1: 6-FAM-CTGAGAGGAAGGTCCCCCACATTGGA-TAMRA | Haugland et al. (2010) |
| Human-associated Bacteroidales (BacHum) | BacHum-160f: TGAGTTCACATGTCCGCATGA BacHum-241r: CGTTACCCCGCCTACTATCTAATG BacHum-193p: 6-FAM-TCCGGTAGACGATGGGGATGCGTT-TAMRA | Kildare et al. (2007) |
| E. coli (EC23S857) | Forward: GGTAGAGCACTGTTTtGGCAa Reverse: TGTCTCCCGTGATAACtTTCTCa Probe: 6-FAM-TCATCCCGACTTACCAACCCG-TAMRA | Chern et al. (2011) |
| Assay . | Primer and probe sequences (5′–3′) . | Reference . |
|---|---|---|
| Human mtDNA | Forward: CAGCAGCCATTCAAGCAATGC Reverse: GGTGGAGACCTAATTGGGCTGATTAG Probe: TATCGGCGATATCGGTTTCATCCTCG | Caldwell et al. (2007) |
| Human-associated Bacteroidales (HF183) | HF183-1: ATCATGAGTTCACATGTCCG BthetR1: CGTAGGAGTTTGGACCGTGT BthetP1: 6-FAM-CTGAGAGGAAGGTCCCCCACATTGGA-TAMRA | Haugland et al. (2010) |
| Human-associated Bacteroidales (BacHum) | BacHum-160f: TGAGTTCACATGTCCGCATGA BacHum-241r: CGTTACCCCGCCTACTATCTAATG BacHum-193p: 6-FAM-TCCGGTAGACGATGGGGATGCGTT-TAMRA | Kildare et al. (2007) |
| E. coli (EC23S857) | Forward: GGTAGAGCACTGTTTtGGCAa Reverse: TGTCTCCCGTGATAACtTTCTCa Probe: 6-FAM-TCATCCCGACTTACCAACCCG-TAMRA | Chern et al. (2011) |
6-FAM, 6-carboxyfluorescein, fluorescence reporter dye; TAMRA, 6-carboxytetramethylrhodamine, fluorescence quencher dye.
aLower case ‘t’ denotes deliberately a mismatched base.