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Table 3

Recovery and robustness of the entire on-site qPCR system in tap water, distilled water and HVAC matrix

Water sourceBacterial input (GU/mL)Mean quantification (GU/mL)Standard deviation of quantificationMean quantification (Log GU/mL)Log SD
Distilled water 20 (n = 16) 17.48 12.72 1.15 0.29 
100 (n = 17) 93.77 42.74 1.94 0.18 
250 (n = 16) 200.88 72.46 2.27 0.17 
Tap water 20 (n = 16) 16.99 8.05 1.18 0.23 
100 (n = 15) 84.90 19.19 1.92 0.10 
250 (n = 18) 201.27 46.37 2.29 0.10 
HVAC 1 20 (n = 10) 15.16 7.33 1.13 0.23 
100 (n = 10) 70.34 25.95 1.82 0.18 
250 (n = 10) 187.55 80.73 2.24 0.18 
HVAC 2 20 (n = 7) 23.80 12.28 1.33 0.21 
100 (n = 9) 103.87 30.99 2.00 0.13 
250 (n = 8) 277.28 70.99 2.43 0.12 
HVAC 3 20 (n = 3) 34.03 9.49 1.52 0.13 
100 (n = 5) 74.29 15.80 1.86 0.09 
250 (n = 5) 255.00 87.85 2.39 0.15 
Water sourceBacterial input (GU/mL)Mean quantification (GU/mL)Standard deviation of quantificationMean quantification (Log GU/mL)Log SD
Distilled water 20 (n = 16) 17.48 12.72 1.15 0.29 
100 (n = 17) 93.77 42.74 1.94 0.18 
250 (n = 16) 200.88 72.46 2.27 0.17 
Tap water 20 (n = 16) 16.99 8.05 1.18 0.23 
100 (n = 15) 84.90 19.19 1.92 0.10 
250 (n = 18) 201.27 46.37 2.29 0.10 
HVAC 1 20 (n = 10) 15.16 7.33 1.13 0.23 
100 (n = 10) 70.34 25.95 1.82 0.18 
250 (n = 10) 187.55 80.73 2.24 0.18 
HVAC 2 20 (n = 7) 23.80 12.28 1.33 0.21 
100 (n = 9) 103.87 30.99 2.00 0.13 
250 (n = 8) 277.28 70.99 2.43 0.12 
HVAC 3 20 (n = 3) 34.03 9.49 1.52 0.13 
100 (n = 5) 74.29 15.80 1.86 0.09 
250 (n = 5) 255.00 87.85 2.39 0.15 
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