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Table 1

Comparison of bacteriological quality and bacterial challenge study; physicochemical quality parameters of control and solar pasteurization treated water samples (pond, lake and river) with standard valuea

Bacteriological qualityNon-treated water samples (as control)
Treated water samples
USEPA std for drinking waterBSTI std for drinking water
indicatorsPondLakeRiverPondLakeRiver(log CFU/ml)(log CFU/ml)
 Mean value of bacterial populations (log CFU/ml)   
Environmental samples 
Total aerobic bacterial count 4.8 ± 0.4 4.1 ± 0.3 4.5 ± 0.1 1.3 ± 0.1 2.6 ± 0.2 0.8 ± 0.1 TT3 1,000 
Total coliform count 4.20 ± 0.1 3.8 ± 0.2 3.8 ± 0.2 <1.0 <1.0 <1.0 NR NR 
E. coli count 3.8 ± 0.1 3.6 ± 0.1 3.1 ± 0.2 <1.0 <1.0 <1.0 NR NR 
E. coli O157:H7 count 3.4 ± 0.1 3.5 ± 0.1 <1.0 <1.0 <1.0 <1.0 NR NR 
Salmonella count <1.0 <1.0 <1.0 <1.0 <1.0 <1.0 NR NR 
Vibrio count <1.0 <1.0 <1.0 <1.0 <1.0 <1.0 NR NR 
Bacterial challenge test 
E. coli on TSAR 5.5 ± 0.3 4.5 ± 0.4 6.8 ± 0.2 <1.0 <1.0 <1.0 NR NR 
E. coli on SMACR 5.3 ± 0.2 4.4 ± 0.2 6.7 ± 0.1 <1.0 <1.0 <1.0 NR NR 
Physicochemical parameters
ParametersMean value of control samples
Mean value of treated samples
US EPA standard for drinking waterBDS standard
Color Gray to yellowish (16–19 color unit) 15 (color unit) 15 (color unit) 5 (Hazen unit) 
Odor Odorous Odorless 3 threshold odor number Unobjectionable 
Taste Fishy-sour smelly Agreeable Agreeable Agreeable 
pH 6.9–7.7 7.1–7.6 6.5–8.5 6.4–7.4 
Salinity (ppt) 0.1 0.1 0.1 0.1 
Turbidity (NTU) 29.0–31.0 5.0–6.0 0.5–1.00 5.0 
TDS 302–346 310–345 500 500 
Conductivity μs/cm 600–780 620–690 NS NS 
BOD (mg/l) 2.1–2.5 2.71–2.76 5.0 5.0 
COD (mg/l) 2.214–3.195 2.27–2.31 40 40 
Iron (mg/L) 0.04 0.04 0.3 0.30 
Manganese (mg/L) 0.02 0.02 0.05 0.50 
Zinc (mg/L) 0.01 0.01 5.00 3.00 
Lead (mg/L) 0.000 0.0004 0.015 0.01 
Arsenic (mg/L) 0.002–0.003 0.002 0.01 0.05 
Cadmium (mg/L) 0.00 0.00 0.005 0.003 
Bacteriological qualityNon-treated water samples (as control)
Treated water samples
USEPA std for drinking waterBSTI std for drinking water
indicatorsPondLakeRiverPondLakeRiver(log CFU/ml)(log CFU/ml)
 Mean value of bacterial populations (log CFU/ml)   
Environmental samples 
Total aerobic bacterial count 4.8 ± 0.4 4.1 ± 0.3 4.5 ± 0.1 1.3 ± 0.1 2.6 ± 0.2 0.8 ± 0.1 TT3 1,000 
Total coliform count 4.20 ± 0.1 3.8 ± 0.2 3.8 ± 0.2 <1.0 <1.0 <1.0 NR NR 
E. coli count 3.8 ± 0.1 3.6 ± 0.1 3.1 ± 0.2 <1.0 <1.0 <1.0 NR NR 
E. coli O157:H7 count 3.4 ± 0.1 3.5 ± 0.1 <1.0 <1.0 <1.0 <1.0 NR NR 
Salmonella count <1.0 <1.0 <1.0 <1.0 <1.0 <1.0 NR NR 
Vibrio count <1.0 <1.0 <1.0 <1.0 <1.0 <1.0 NR NR 
Bacterial challenge test 
E. coli on TSAR 5.5 ± 0.3 4.5 ± 0.4 6.8 ± 0.2 <1.0 <1.0 <1.0 NR NR 
E. coli on SMACR 5.3 ± 0.2 4.4 ± 0.2 6.7 ± 0.1 <1.0 <1.0 <1.0 NR NR 
Physicochemical parameters
ParametersMean value of control samples
Mean value of treated samples
US EPA standard for drinking waterBDS standard
Color Gray to yellowish (16–19 color unit) 15 (color unit) 15 (color unit) 5 (Hazen unit) 
Odor Odorous Odorless 3 threshold odor number Unobjectionable 
Taste Fishy-sour smelly Agreeable Agreeable Agreeable 
pH 6.9–7.7 7.1–7.6 6.5–8.5 6.4–7.4 
Salinity (ppt) 0.1 0.1 0.1 0.1 
Turbidity (NTU) 29.0–31.0 5.0–6.0 0.5–1.00 5.0 
TDS 302–346 310–345 500 500 
Conductivity μs/cm 600–780 620–690 NS NS 
BOD (mg/l) 2.1–2.5 2.71–2.76 5.0 5.0 
COD (mg/l) 2.214–3.195 2.27–2.31 40 40 
Iron (mg/L) 0.04 0.04 0.3 0.30 
Manganese (mg/L) 0.02 0.02 0.05 0.50 
Zinc (mg/L) 0.01 0.01 5.00 3.00 
Lead (mg/L) 0.000 0.0004 0.015 0.01 
Arsenic (mg/L) 0.002–0.003 0.002 0.01 0.05 
Cadmium (mg/L) 0.00 0.00 0.005 0.003 

aDetection limit: <1.0 log CFU/ml. TT3: treatment technique, a required process intended to reduce the level of contamination in drinking water; NR: not recommended; TSAR: tryptic soy agar with rifampicin (50 μg/ml); SMACR: sorbitol MacConkey agar with rifampicin (50 μg/ml); NS: not specified.

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