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Table 2

Chromatographic conditions

ParameterDescription
Instrument Agilent 1200 Series HPLC 
Injection volume 10 μL 
Column temperature 25 °C 
Column Waters Symmetry® C18, 250 × 4.6 mm, 5 μm 
Flow rate 1 mL/min 
Detector Diode Array Detector (HPLC-DAD) 
Mobile phase A: Acetonitrile 70% B: DI water 30% 
Wavelength 273 nm 
ParameterDescription
Instrument Agilent 1200 Series HPLC 
Injection volume 10 μL 
Column temperature 25 °C 
Column Waters Symmetry® C18, 250 × 4.6 mm, 5 μm 
Flow rate 1 mL/min 
Detector Diode Array Detector (HPLC-DAD) 
Mobile phase A: Acetonitrile 70% B: DI water 30% 
Wavelength 273 nm 
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