Primer sequences of the relative resistance genes and reaction conditions
Genes . | Primer sequences(5′–3′) . | Annealing temperature (°C) . | Length of fragment (bp) . |
---|---|---|---|
tetA | F:GCTACATCCTGCTTGCCTTC | 55 | 210 |
R:CATAGATCGCCGTGAAGAGG | |||
tetB | F:TACGTGAATTTATTGCTTCGG | 61 | 206 |
R:ATACAGCATCCAAAGCGCAC | |||
tetG | F:GCACGCTGGTTTGGCTACA | 56 | 176 |
R:TGGCTGTGATTAGTCTCCTTGA | |||
tetM | F:ACAGAAAGCTTATTATATAAC | 45 | 171 |
R:TGGCGTGTCTATGATGTTCAC | |||
sul1 | F:CGCACCGGAAACATCGCTGCAC | 56 | 163 |
R:TGAAGTTCCGCCGCAAGGCTCG | |||
sul2 | F:TCCGGTGGAGGCCGGTATCTGG | 56 | 191 |
R:CGGGAATGCCATCTGCCTTGAG | |||
sul3 | F:CCCATACCCGGATCAAGAATAA | 57 | 143 |
R:CAGCGAATTGGTGCAGCTACTA | |||
blaTEM | F:AAGCTTATTAGCGTGT | 55 | 531 |
R:TGTCTATGATCTTGT | |||
blaCTX-M | F:GCTGGTTTCAGAA | 55 | 869 |
R:TGTGATTAGCTGT | |||
blaSHV | F:TGAATACGTTTTCAC | 55 | 295 |
R:TACCTTGTTATTCAC | |||
intI | F:GGCTTCGTGATGCCTGCTT | 57 | 146 |
R:CATTCCTGGCCGTGGTTCT | |||
16S rDNA | 27F:AGAGTTTGATCCTGCCTCAG | 56 | 1465 |
1492R:TACGGYTACCTTGTTACGACTT |
Genes . | Primer sequences(5′–3′) . | Annealing temperature (°C) . | Length of fragment (bp) . |
---|---|---|---|
tetA | F:GCTACATCCTGCTTGCCTTC | 55 | 210 |
R:CATAGATCGCCGTGAAGAGG | |||
tetB | F:TACGTGAATTTATTGCTTCGG | 61 | 206 |
R:ATACAGCATCCAAAGCGCAC | |||
tetG | F:GCACGCTGGTTTGGCTACA | 56 | 176 |
R:TGGCTGTGATTAGTCTCCTTGA | |||
tetM | F:ACAGAAAGCTTATTATATAAC | 45 | 171 |
R:TGGCGTGTCTATGATGTTCAC | |||
sul1 | F:CGCACCGGAAACATCGCTGCAC | 56 | 163 |
R:TGAAGTTCCGCCGCAAGGCTCG | |||
sul2 | F:TCCGGTGGAGGCCGGTATCTGG | 56 | 191 |
R:CGGGAATGCCATCTGCCTTGAG | |||
sul3 | F:CCCATACCCGGATCAAGAATAA | 57 | 143 |
R:CAGCGAATTGGTGCAGCTACTA | |||
blaTEM | F:AAGCTTATTAGCGTGT | 55 | 531 |
R:TGTCTATGATCTTGT | |||
blaCTX-M | F:GCTGGTTTCAGAA | 55 | 869 |
R:TGTGATTAGCTGT | |||
blaSHV | F:TGAATACGTTTTCAC | 55 | 295 |
R:TACCTTGTTATTCAC | |||
intI | F:GGCTTCGTGATGCCTGCTT | 57 | 146 |
R:CATTCCTGGCCGTGGTTCT | |||
16S rDNA | 27F:AGAGTTTGATCCTGCCTCAG | 56 | 1465 |
1492R:TACGGYTACCTTGTTACGACTT |