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Table 2

Preliminary assay with CCF and DC+ICN 7X detection protocols, followed by IMS with two acid dissociations to compare the recovery of Giardia spp. cysts artificially inoculated in the FBW

Recovery from detection protocolsNumber of cysts recovered
Recovery (%)
First dissociationSecond dissociationTotalFirst dissociationSecond dissociationTotal
CCF+IMS 83 74 157 7.6 6.8 14.4 
DC+ICN 7X+IMS 60 43 103 6.2 4.5 10.7 
PI-negative cysts from detection protocolsNumber of PI-negative cysts recovered
PI-negative cysts from the recovered (%)
First dissociationSecond dissociationTotalFirst dissociationSecond dissociationTotal
CCF+IMS 15 28 43 18.1 37.8 27.4 
DC+ICN 7X+IMS 59 27 86 98.3 62.8 83.5 
Recovery from detection protocolsNumber of cysts recovered
Recovery (%)
First dissociationSecond dissociationTotalFirst dissociationSecond dissociationTotal
CCF+IMS 83 74 157 7.6 6.8 14.4 
DC+ICN 7X+IMS 60 43 103 6.2 4.5 10.7 
PI-negative cysts from detection protocolsNumber of PI-negative cysts recovered
PI-negative cysts from the recovered (%)
First dissociationSecond dissociationTotalFirst dissociationSecond dissociationTotal
CCF+IMS 15 28 43 18.1 37.8 27.4 
DC+ICN 7X+IMS 59 27 86 98.3 62.8 83.5 

CCF, calcium carbonate flocculation; DC, direct centrifugation; ICN 7X, ICN detergent dispersion solution 7X; IMS, immunomagnetic separation.

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