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Table 3

Average recoveries and coefficient of variation for the analytical quality assays with Giardia spp. suspensions detected by the DC+ICN 7X+IMS protocol for the FBW, considering stained (S) (PI-positive) and not-stained (NS) (PI-negative) cysts

SamplesCommercial suspension (inoculated cysts in the FBW: 1,900 cysts L−1)
First dissociation
Second dissociation
Total number
ReplicatesNSSNSSNSSTotal recovery (%)Non-stained cysts (%)
72 159 25 117 97 276 19.6 26.0 
102 167 11 101 113 268 20.1 29.7 
65 143 18 132 83 275 18.8 23.2 
Average 79.7 156.3 18.0 116.7 97.7 273.0 19.5 26.0 
Standard deviation 19.7 12.2 7.0 15.5 15.0 4.4 0.7 3.3 
Coefficient of variation 24.7 7.8 38.9 13.3 15.4 1.6 3.4 12.4 
EasySeed® suspension (inoculated cysts in the FBW: 100.0±1.9 cysts L−1)
SamplesFirst dissociation
Second dissociation
Total number
Total recovery (%)
ReplicatesS
S
S
 8.0 
 7.0 
 4.0 
10 12  12.0 
Average 4.5 3.3 7.8  7.8 
Standard deviation 3.2 1.8 2.9  2.9 
Coefficient of variation 71.1 54.9 36.9  36.9 
SamplesCommercial suspension (inoculated cysts in the FBW: 1,900 cysts L−1)
First dissociation
Second dissociation
Total number
ReplicatesNSSNSSNSSTotal recovery (%)Non-stained cysts (%)
72 159 25 117 97 276 19.6 26.0 
102 167 11 101 113 268 20.1 29.7 
65 143 18 132 83 275 18.8 23.2 
Average 79.7 156.3 18.0 116.7 97.7 273.0 19.5 26.0 
Standard deviation 19.7 12.2 7.0 15.5 15.0 4.4 0.7 3.3 
Coefficient of variation 24.7 7.8 38.9 13.3 15.4 1.6 3.4 12.4 
EasySeed® suspension (inoculated cysts in the FBW: 100.0±1.9 cysts L−1)
SamplesFirst dissociation
Second dissociation
Total number
Total recovery (%)
ReplicatesS
S
S
 8.0 
 7.0 
 4.0 
10 12  12.0 
Average 4.5 3.3 7.8  7.8 
Standard deviation 3.2 1.8 2.9  2.9 
Coefficient of variation 71.1 54.9 36.9  36.9 

DC, direct centrifugation; ICN 7X, ICN detergent dispersion solution 7X; IMS, immunomagnetic separation.

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