By simulating a PNA MABR under different influent flow rates and total ammonia concentrations, the performance of the reactor can be demonstrated under numerous nitrogen loadings and concentrations which is essential for determining how many reactors are needed to treat a given wastewater flow. From these simulations, we observed that increasing the flow rate to the MABR decreased its HRT and increased the nitrogen loading rate for each influent total ammonia concentration tested. As the nitrogen loading rate increased for every influent ammonia-nitrogen concentration, the TIN removal percentage decreased and never increased, Figure 2 (bottom). This decrease in TIN removal percentage was abrupt for lower influent total ammonia concentrations, occurring at loading rates less than 3 g N/m²/d for influent ammonia concentration less than 50 mg N/L, and more gradual for larger influent concentrations. When comparing the reactor's performance against the stoichiometric nitrogen removal for AOO and AMX, the TIN removal percentage never reached the theoretical removal percentage of 89% for any nitrogen loading rate or influent concentration because the biofilm was loaded with oxygen below the stoichiometric oxygen demand for PNA, 1.75 g O₂/g N (Strous et al. 1998), to create oxygen-limiting conditions to prevent NOB growth. The effluent TIN concentration also increased with increased nitrogen loading rates (Figure S-2). These findings are unsurprising and can be observed in other biofilm-based reactors such as rotating biological contactors and packed towers.