The design methodology produced an example treatment train with 35.5% of its membrane surface area in each of the first two stages and 29% in the last stage. Effluent total nitrogen, total ammonia, nitrite, and nitrate concentrations for the designed treatment train are shown in Table 2. The design was calculated to have an effluent total nitrogen concentration of 3.5mg N/L and a total ammonia concentration of 0.9mg N/L. 76% of the total nitrogen calculated to be removed in the entire treatment train occurred in the first stage since it had the largest loading and largest effluent ammonia concentration, seen in Table 2, which promoted mass transfer into the biofilm and increased the specific growth rates of AOO and AMX. The first two stages were calculated to have a 71% TIN removal of their influent total ammonia, but the second stage had a smaller mass removal due to the lower loading applied to the reactor and removed 19% of the train's total nitrogen. The last reactor had the smallest total ammonia loading and had an influent total ammonia concentration of only 3.1mg N/L. Since the effluent total ammonia concentration was required to be below 1mg N/L, the total ammonia concentration was the limiting factor for the design, and the last stage was sized just large enough to reach the effluent limit. As a result, the last stage only removed 5% of the train's total nitrogen loading and was used primarily for total ammonia oxidation.