Visual analysis of the average decay rates of individual samples, combined with an estimated 95% CIs, indicated a high degree of overlap between individual samples and all three targets at both 25 and 35 °C (Supplementary material, Figures S2 and S3). Visual analysis of the pooled experimental data indicated a high degree of overlap between our three RNA targets, indicating that all targets degrade at approximately the same rate at both test temperatures (Figure 2). The average decay rates of pooled experiments at 25 °C for E, N1, and N2 were 0.0094, 0.0116, and 0.0117 h−1, respectively (Table 3). The average decay rate of pooled samples at 35 °C were E, N1, and N2 were 0.0193, 0.0157, and 0.0134 h−1, respectively (Table 4). Results of individual experiments, including decay rate k, half-life T50, and time T90 to 90% decay, are provided in the supplemental material (Supplementary material, Tables S2 and S3).
Table 3

Average decay rate, half-life T50, and time T90 to 90% decay for E, N1, and N2 at 25 °C for six independent experiments

Markerk, h−1T50, hT90, h
0.0094 (0.0057–0.0131) 102 (69–257) 338 (228–853) 
N1 0.0116 (0.0065–0.0167) 71 (50–176) 236 (165–585) 
N2 0.0117 (0.0050–0.0163) 74 (49–247) 246 (162–821) 
Markerk, h−1T50, hT90, h
0.0094 (0.0057–0.0131) 102 (69–257) 338 (228–853) 
N1 0.0116 (0.0065–0.0167) 71 (50–176) 236 (165–585) 
N2 0.0117 (0.0050–0.0163) 74 (49–247) 246 (162–821) 

Average values of confidence intervals for the six experiments are shown in parentheses.

Table 4

Average decay rate, half-life, and T90 for E, N1 and N2 at 35 °C for six independent experiments

Markerk, h−1T50, hT90, h
0.0193 (0.0103–0.0284) 42 (29–80) 138 (95–266) 
N1 0.0157 (0.0067–0.0248) 54 (35–172) 180 (115–570) 
N2 0.0134 (0.0058–0.0211) 64 (40–276) 214 (133–917) 
Markerk, h−1T50, hT90, h
0.0193 (0.0103–0.0284) 42 (29–80) 138 (95–266) 
N1 0.0157 (0.0067–0.0248) 54 (35–172) 180 (115–570) 
N2 0.0134 (0.0058–0.0211) 64 (40–276) 214 (133–917) 

Average values of confidence intervals are shown in parentheses.

Figure 2

Average decay rate for N1 (square), N2 (triangle), and E (circle) at 25 and 35 °C for six independent experiments. The average 95% confidence interval of all experiments is also plotted (shaded bar).

Figure 2

Average decay rate for N1 (square), N2 (triangle), and E (circle) at 25 and 35 °C for six independent experiments. The average 95% confidence interval of all experiments is also plotted (shaded bar).

Close modal
Close Modal

or Create an Account

Close Modal
Close Modal