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Table 1 shows that the effectiveness of reducing bacterial growth by Puristic is not significant and tends to increase. This can be caused by the possibility of contamination from around the incubator and the ability of bacteria can grow or inactivate in a room environment. Table 1 is a resume of the results of the Puristic test at 37 °C which has been carried out in the laboratory and has been facilitated without plastic as can be seen in Figure 1(b).

Table 1

Results of the disinfectant at an incubator temperature of 37 °C

Temperature	Gram staining	Bacteria	Groups	Puristic		Virus Shut Out
Temperature	Gram staining	Bacteria	Groups	Numbers (CFU/mL)	Growth inhibition rate (%)	Numbers (CFU/mL)	Growth inhibition rate (%)
37 °C	+	Staphylococcus aureus	Disinfectant (ClO₂)	5.0 × 10³	99	1.1 × 10⁷	9
	+	Staphylococcus aureus	Control	1.1 × 10⁷	99	1.0 × 10⁷	9
	+	Staphylococcus epidermidis	Disinfectant (ClO₂)	3.5 × 10⁶	29	3.0 × 10⁶	–
	+	Staphylococcus epidermidis	Control	4.9 × 10⁶	29	3.2 × 10⁶	–
	+	Bacillus subtilis	Disinfectant (ClO₂)	6.7 × 10²	99	2.7 × 10⁶	30
	+	Bacillus subtilis	Control	7.0 × 10⁵	99	1.9 × 10⁶	30
	+	Enterococcus faecalis	Disinfectant (ClO₂)	2.9 × 10⁶	83	2.4 × 10⁷	–
	+	Enterococcus faecalis	Control	1.7 × 10⁷	83	2.5 × 10⁷	–
	–	Escherichia coli	Disinfectant (ClO₂)	8.5 × 10⁴	99	2.8 × 10⁶	64
	–	Escherichia coli	Control	1.9 × 10⁷	99	1.0 × 10⁶	64
	–	Salmonella typhimurium	Disinfectant (ClO₂)	1.2 × 10⁴	99	2.7 × 10⁷	7
	–	Salmonella typhimurium	Control	8.1 × 10⁶	99	2.5 × 10⁷	7
	–	Pseudomonas aeruginosa	Disinfectant (ClO₂)	2.1 × 10⁷	5	4.0 × 10⁷	22
	–	Pseudomonas aeruginosa	Control	2.2 × 10⁷	5	3.1 × 10⁷	22
	–	Klebsiella pneumonia	Disinfectant (ClO₂)	1.3 × 10⁷	–	4.1 × 10⁶	17
	–	Klebsiella pneumonia	Control	1.0 × 10⁷	–	3.4 × 10⁶	17
	–	Enterobacter aerogenes	Disinfectant (ClO₂)	>3.0 × 10⁷	–	1.1 × 10⁷	9
	–	Enterobacter aerogenes	Control	>3.0 × 10⁷	–	1.0 × 10⁷	9

Temperature	Gram staining	Bacteria	Groups	Puristic		Virus Shut Out
Temperature	Gram staining	Bacteria	Groups	Numbers (CFU/mL)	Growth inhibition rate (%)	Numbers (CFU/mL)	Growth inhibition rate (%)
37 °C	+	Staphylococcus aureus	Disinfectant (ClO₂)	5.0 × 10³	99	1.1 × 10⁷	9
	+	Staphylococcus aureus	Control	1.1 × 10⁷	99	1.0 × 10⁷	9
	+	Staphylococcus epidermidis	Disinfectant (ClO₂)	3.5 × 10⁶	29	3.0 × 10⁶	–
	+	Staphylococcus epidermidis	Control	4.9 × 10⁶	29	3.2 × 10⁶	–
	+	Bacillus subtilis	Disinfectant (ClO₂)	6.7 × 10²	99	2.7 × 10⁶	30
	+	Bacillus subtilis	Control	7.0 × 10⁵	99	1.9 × 10⁶	30
	+	Enterococcus faecalis	Disinfectant (ClO₂)	2.9 × 10⁶	83	2.4 × 10⁷	–
	+	Enterococcus faecalis	Control	1.7 × 10⁷	83	2.5 × 10⁷	–
	–	Escherichia coli	Disinfectant (ClO₂)	8.5 × 10⁴	99	2.8 × 10⁶	64
	–	Escherichia coli	Control	1.9 × 10⁷	99	1.0 × 10⁶	64
	–	Salmonella typhimurium	Disinfectant (ClO₂)	1.2 × 10⁴	99	2.7 × 10⁷	7
	–	Salmonella typhimurium	Control	8.1 × 10⁶	99	2.5 × 10⁷	7
	–	Pseudomonas aeruginosa	Disinfectant (ClO₂)	2.1 × 10⁷	5	4.0 × 10⁷	22
	–	Pseudomonas aeruginosa	Control	2.2 × 10⁷	5	3.1 × 10⁷	22
	–	Klebsiella pneumonia	Disinfectant (ClO₂)	1.3 × 10⁷	–	4.1 × 10⁶	17
	–	Klebsiella pneumonia	Control	1.0 × 10⁷	–	3.4 × 10⁶	17
	–	Enterobacter aerogenes	Disinfectant (ClO₂)	>3.0 × 10⁷	–	1.1 × 10⁷	9
	–	Enterobacter aerogenes	Control	>3.0 × 10⁷	–	1.0 × 10⁷	9

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Table 2

Results of the disinfectant at an incubator temperature of 30 °C

Temperature	Gram staining	Bacteria	Groups	Puristic		Virus Shut Out
Temperature	Gram staining	Bacteria	Groups	Numbers (CFU/mL)	Growth inhibition rate (%)	Numbers (CFU/mL)	Growth inhibition rate (%)
30 °C	+	Staphylococcus aureus	Disinfectant (ClO₂)	3.5 × 10⁶	41	1.1 × 10⁷	–
	+	Staphylococcus aureus	Control	5.9 × 10⁶	41	1.1 × 10⁷	–
	+	Staphylococcus epidermidis	Disinfectant (ClO₂)	4.0 × 10⁶	–	4.1 × 10⁶	22
	+	Staphylococcus epidermidis	Control	3.6 × 10⁶	–	3.2 × 10⁶	22
	+	Bacillus subtilis	Disinfectant (ClO₂)	1.5 × 10⁶	66	2.6 × 10⁶	23
	+	Bacillus subtilis	Control	4.4 × 10⁶	66	2.0 × 10⁶	23
	+	Enterococcus faecalis	Disinfectant (ClO₂)	3.5 × 10⁶	35	2.7 × 10⁷	22
	+	Enterococcus faecalis	Control	5.4 × 10⁶	35	2.1 × 10⁷	22
	–	Escherichia coli	Disinfectant (ClO₂)	2.0 × 10⁵	96	1.8 × 10⁶	22
	–	Escherichia coli	Control	4.9 × 10⁶	96	1.4 × 10⁶	22
	–	Salmonella typhimurium	Disinfectant (ClO₂)	1.6 × 10⁷	20	3.4 × 10⁷	38
	–	Salmonella typhimurium	Control	2.0 × 10⁷	20	2.1 × 10⁷	38
	–	Pseudomonas aeruginosa	Disinfectant (ClO₂)	2.6 × 10⁷	–	3.1 × 10⁷	13
	–	Pseudomonas aeruginosa	Control	1.9 × 10⁷	–	2.7 × 10⁷	13
	–	Klebsiella pneumonia	Disinfectant (ClO₂)	8.8 × 10⁶	27	4.1 × 10⁶	17
	–	Klebsiella pneumonia	Control	1.2 × 10⁷	27	3.4 × 10⁶	17
	–	Enterobacter aerogenes	Disinfectant (ClO₂)	1.7 × 10⁷	–	1.1 × 10⁷	–
	–	Enterobacter aerogenes	Control	1.5 × 10⁷	–	1.1 × 10⁷	–

Temperature	Gram staining	Bacteria	Groups	Puristic		Virus Shut Out
Temperature	Gram staining	Bacteria	Groups	Numbers (CFU/mL)	Growth inhibition rate (%)	Numbers (CFU/mL)	Growth inhibition rate (%)
30 °C	+	Staphylococcus aureus	Disinfectant (ClO₂)	3.5 × 10⁶	41	1.1 × 10⁷	–
	+	Staphylococcus aureus	Control	5.9 × 10⁶	41	1.1 × 10⁷	–
	+	Staphylococcus epidermidis	Disinfectant (ClO₂)	4.0 × 10⁶	–	4.1 × 10⁶	22
	+	Staphylococcus epidermidis	Control	3.6 × 10⁶	–	3.2 × 10⁶	22
	+	Bacillus subtilis	Disinfectant (ClO₂)	1.5 × 10⁶	66	2.6 × 10⁶	23
	+	Bacillus subtilis	Control	4.4 × 10⁶	66	2.0 × 10⁶	23
	+	Enterococcus faecalis	Disinfectant (ClO₂)	3.5 × 10⁶	35	2.7 × 10⁷	22
	+	Enterococcus faecalis	Control	5.4 × 10⁶	35	2.1 × 10⁷	22
	–	Escherichia coli	Disinfectant (ClO₂)	2.0 × 10⁵	96	1.8 × 10⁶	22
	–	Escherichia coli	Control	4.9 × 10⁶	96	1.4 × 10⁶	22
	–	Salmonella typhimurium	Disinfectant (ClO₂)	1.6 × 10⁷	20	3.4 × 10⁷	38
	–	Salmonella typhimurium	Control	2.0 × 10⁷	20	2.1 × 10⁷	38
	–	Pseudomonas aeruginosa	Disinfectant (ClO₂)	2.6 × 10⁷	–	3.1 × 10⁷	13
	–	Pseudomonas aeruginosa	Control	1.9 × 10⁷	–	2.7 × 10⁷	13
	–	Klebsiella pneumonia	Disinfectant (ClO₂)	8.8 × 10⁶	27	4.1 × 10⁶	17
	–	Klebsiella pneumonia	Control	1.2 × 10⁷	27	3.4 × 10⁶	17
	–	Enterobacter aerogenes	Disinfectant (ClO₂)	1.7 × 10⁷	–	1.1 × 10⁷	–
	–	Enterobacter aerogenes	Control	1.5 × 10⁷	–	1.1 × 10⁷	–

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