From Table 3, it can be seen that bacterial growth is inhibited if it does not reach the optimum temperature (37 °C). This growth is further hampered by the presence of Puristic and Virus Shut Out that act as disinfectants. Even though the Puristic's disinfection process was not as significant as it was at 37 °C, the decrease in bacterial growth in this experiment occurred in almost all the bacteria used. From this experiment, it can also be seen that the performance of Puristic and Virus Shut Out as a disinfectant was affected by the temperature and the content of the disinfectant itself.

Table 3

Comparison of growth inhibition rates

BacteriaPuristic
Virus Shut Out
37 °C30 °C37 °C30 °C
Staphylococcus aureus 99% 41% 9% – 
Staphylococcus epidermidis 29% – – 22% 
Bacillus subtilis 99% 66% 30% 23% 
Enterococcus faecalis 83% 35% – 22% 
Escherichia coli 99% 96% 64% 22% 
Salmonella typhimurium 99% 20% 7% 38% 
Pseudomonas aeruginosa 5% – 22% 13% 
Klebsiella pneumonia – 27% 17% 17% 
Enterobacter aerogenes – – 9% – 
BacteriaPuristic
Virus Shut Out
37 °C30 °C37 °C30 °C
Staphylococcus aureus 99% 41% 9% – 
Staphylococcus epidermidis 29% – – 22% 
Bacillus subtilis 99% 66% 30% 23% 
Enterococcus faecalis 83% 35% – 22% 
Escherichia coli 99% 96% 64% 22% 
Salmonella typhimurium 99% 20% 7% 38% 
Pseudomonas aeruginosa 5% – 22% 13% 
Klebsiella pneumonia – 27% 17% 17% 
Enterobacter aerogenes – – 9% – 
Table 4

Indoor test results of Puristic

TreatmentExposure time (h)Distance (m)Number of colonyGrowth inhibition rate (%)
Control without Puristic 24 – 33 – 
With Puristic <1 15 54.55 
<1 18 45.45 
24 <1 10 69.69 
48 <1 11 66.67 
TreatmentExposure time (h)Distance (m)Number of colonyGrowth inhibition rate (%)
Control without Puristic 24 – 33 – 
With Puristic <1 15 54.55 
<1 18 45.45 
24 <1 10 69.69 
48 <1 11 66.67 

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