A study to verify UDD antimicrobial properties was achieved by the treatment of polluted water with different UDD concentrations. Table 1 shows UDD-treated water fecal E. coli CFU and control polluted comparison at different time intervals. Results indicated a significant reduction of CFU when polluted water was mixed with UDD. At 0 min, the control water and UDD-treated water showed fecal E. coli bacteria concentrations of 950 ± 100 and 875 ± 98 Col/cm3, respectively. After 40 min, control plates had a sample mean of 2,425 ± 96 Col/cm3 and UDD-treated water plates had a sample mean of 150 ± 57 Col/cm3. Fecal coliforms death rate results for each time interval showed a constant reduction of CFU as time passed.

Table 1

Fecal E. coli CFU and CV % for polluted water and UDD-treated water with UDD petri dish samples

UDD (g/cm3)Time (min)Sample mean untreated H2O (Col/cm3)CV %Sample mean UDD treated H2O (Col/cm3)CV %Death rate %
0.01 0 950 ± 100 1 875 ± 96 11 7 
10 1,225 ± 126 10 525 ± 95 18 58 
20 1,750 ± 129 7 500 ± 58 11 71 
30 2,175 ± 170 8 275 ± 50 18 87 
40 2,425 ± 96 4 150 ± 57 38 94 
0.02 0 600 ± 0 0 300 ± 100 33 50 
10 733 ± 208 28 233 ± 58 50 68 
20 833 ± 153 18 200 ± 100 50 76 
30 1,000 ± 100 10 133 ± 58 43 87 
40 1,166 ± 58 5 33±58 117 97 
UDD (g/cm3)Time (min)Sample mean untreated H2O (Col/cm3)CV %Sample mean UDD treated H2O (Col/cm3)CV %Death rate %
0.01 0 950 ± 100 1 875 ± 96 11 7 
10 1,225 ± 126 10 525 ± 95 18 58 
20 1,750 ± 129 7 500 ± 58 11 71 
30 2,175 ± 170 8 275 ± 50 18 87 
40 2,425 ± 96 4 150 ± 57 38 94 
0.02 0 600 ± 0 0 300 ± 100 33 50 
10 733 ± 208 28 233 ± 58 50 68 
20 833 ± 153 18 200 ± 100 50 76 
30 1,000 ± 100 10 133 ± 58 43 87 
40 1,166 ± 58 5 33±58 117 97 
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