The chloride content in the control sample (MSM or STWW + Ps. putida AE015451) significantly increased at TF compared to T0 (Table 2). Different experimental conditions were studied such as PCP, Fe content, inoculum volume, and pH of the matrix. Karn et al. (2010a) proved that the PCP used and the discharge of chloride with inside the medium was essential because of the PCP mineralization.
Chloride concentration (g L−1) at T0 and after 7 days (TF) in the bioaugmentation experiment with Pseudomonas putida AE015451 in MSM and STWW samples under different experimental conditions
| Treatments . | MSM . | STWW . | |||
|---|---|---|---|---|---|
| T0 . | TF . | T0 . | TF . | ||
| Control | – | 0.56 ± 0.01 b | 0.80 ± 0.02 a | 1.25 ± 0.36 b | 3.60 ± 0.35 a |
| PCP (mg L−1) | 30 | 1.36 ± 0.03 Db | 4.20 ± 0.03 Aa | 2.05 ± 0.17 Cb | 3.40 ± 1.04 Ca |
| 50 | 1.98 ± 0.01 Ab | 3.80 ± 0.03 Ba | 2.05 ± 0.16 Cb | 3.20 ± 0.45 Ca | |
| 70 | 1.69 ± 0.06 Bb | 2.80 ± 0.46 Ca | 1.07 ± 0.36 Db | 3.40 ± 0.54 Ca | |
| 100 | 1.33 ± 0.04 Db | 1.80 ± 0.33 Da | 2.36 ± 1.03 Cb | 4.20 ± 0.33 Ba | |
| 200 | 1.50 ± 0.07 Ca | 1.60 ± 1.03 Da | 5.24 ± 3.03 Bb | 14.20 ± 0.25 Aa | |
| 300 | 1.51 ± 0.05 Ca | 1.68 ± 0.99 Da | 8.33 ± 2.04 Ab | 14.00 ± 0.33 Aa | |
| Fe (mg L−1) + 30 mg L−1 PCP | 5 | 0.40 ± 0.03 Bb | 2.35 ± 0.01 Aa | 2.31 ± 0.21 Ab | 3.80 ± 0.03 Aa |
| 10 | 0.52 ± 0.01 Ab | 2.30 ± 0.04 Aa | 2.01 ± 0.36 Ab | 3.00 ± 0.15 Ba | |
| 20 | 0.48 ± 0.03 Ab | 1.95 ± 0.01 Ba | 1.98 ± 0.33 Ab | 3.00 ± 0.28 Ba | |
| Inoculum (mL) + 30 mg L−1 PCP | 0.5 | 0.46 ± 0.01 Bb | 6.20 ± 0.03 Aa | 1.52 ± 0.98 Aa | 2.20 ± 0.03 Ba |
| 1 | 0.65 ± 0.03 Ab | 8.40 ± 0.03 Ba | 1.52 ± 0.65 Aa | 4.40 ± 1.24 Aa | |
| 2 | 0.37 ± 0.04 Cb | 4.60 ± 0.04 Ca | 2.01 ± 0.14 Ab | 2.40 ± 1.10 Ba | |
| pH + 30 mg L−1 PCP | 4 | 0.89 ± 0.06 Db | 1.12 ± 0.66 Ba | 2.01 ± 0.66 Ab | 3.80 ± 0.33 Aa |
| 5 | 0.79 ± 0.06 Db | 3.40 ± 0.99 Aa | 2.04 ± 0.14 Ab | 2.40 ± 0.43 Aa | |
| 6.3 | 1.02 ± 0.04 Cb | 3.20 ± 0.53 Aa | 0.80 ± 0.25 Bb | 2.72 ± 0.43 Ba | |
| 7 | 1.36 ± 0.04 Bb | 2.90 ± 0.04 Aa | 0.98 ± 0.33 Bb | 2.20 ± 0.53 Ba | |
| 8 | 1.45 ± 0.02 Ab | 3.26 ± 0.02 Aa | 1.04 ± 0.99 Bb | 2.40 ± 0.44 Ba | |
| Treatments . | MSM . | STWW . | |||
|---|---|---|---|---|---|
| T0 . | TF . | T0 . | TF . | ||
| Control | – | 0.56 ± 0.01 b | 0.80 ± 0.02 a | 1.25 ± 0.36 b | 3.60 ± 0.35 a |
| PCP (mg L−1) | 30 | 1.36 ± 0.03 Db | 4.20 ± 0.03 Aa | 2.05 ± 0.17 Cb | 3.40 ± 1.04 Ca |
| 50 | 1.98 ± 0.01 Ab | 3.80 ± 0.03 Ba | 2.05 ± 0.16 Cb | 3.20 ± 0.45 Ca | |
| 70 | 1.69 ± 0.06 Bb | 2.80 ± 0.46 Ca | 1.07 ± 0.36 Db | 3.40 ± 0.54 Ca | |
| 100 | 1.33 ± 0.04 Db | 1.80 ± 0.33 Da | 2.36 ± 1.03 Cb | 4.20 ± 0.33 Ba | |
| 200 | 1.50 ± 0.07 Ca | 1.60 ± 1.03 Da | 5.24 ± 3.03 Bb | 14.20 ± 0.25 Aa | |
| 300 | 1.51 ± 0.05 Ca | 1.68 ± 0.99 Da | 8.33 ± 2.04 Ab | 14.00 ± 0.33 Aa | |
| Fe (mg L−1) + 30 mg L−1 PCP | 5 | 0.40 ± 0.03 Bb | 2.35 ± 0.01 Aa | 2.31 ± 0.21 Ab | 3.80 ± 0.03 Aa |
| 10 | 0.52 ± 0.01 Ab | 2.30 ± 0.04 Aa | 2.01 ± 0.36 Ab | 3.00 ± 0.15 Ba | |
| 20 | 0.48 ± 0.03 Ab | 1.95 ± 0.01 Ba | 1.98 ± 0.33 Ab | 3.00 ± 0.28 Ba | |
| Inoculum (mL) + 30 mg L−1 PCP | 0.5 | 0.46 ± 0.01 Bb | 6.20 ± 0.03 Aa | 1.52 ± 0.98 Aa | 2.20 ± 0.03 Ba |
| 1 | 0.65 ± 0.03 Ab | 8.40 ± 0.03 Ba | 1.52 ± 0.65 Aa | 4.40 ± 1.24 Aa | |
| 2 | 0.37 ± 0.04 Cb | 4.60 ± 0.04 Ca | 2.01 ± 0.14 Ab | 2.40 ± 1.10 Ba | |
| pH + 30 mg L−1 PCP | 4 | 0.89 ± 0.06 Db | 1.12 ± 0.66 Ba | 2.01 ± 0.66 Ab | 3.80 ± 0.33 Aa |
| 5 | 0.79 ± 0.06 Db | 3.40 ± 0.99 Aa | 2.04 ± 0.14 Ab | 2.40 ± 0.43 Aa | |
| 6.3 | 1.02 ± 0.04 Cb | 3.20 ± 0.53 Aa | 0.80 ± 0.25 Bb | 2.72 ± 0.43 Ba | |
| 7 | 1.36 ± 0.04 Bb | 2.90 ± 0.04 Aa | 0.98 ± 0.33 Bb | 2.20 ± 0.53 Ba | |
| 8 | 1.45 ± 0.02 Ab | 3.26 ± 0.02 Aa | 1.04 ± 0.99 Bb | 2.40 ± 0.44 Ba | |
Different capital letters show significant differences among the different experimental conditions (Fe concentration, Inoculum volume, PCP concentration, and pH) in the sampling time. The different lowercase letter shows differences between the two sampling times (T0 and TF).