The bacterial biomass in the control sample MSM or STWW + Ps. putida AE015451 showed an increase between T0 and TF (Table 3). Also, different experimental conditions were examined such as PCP, Fe content, bacterial inoculum volume, and pH of the medium.
Bacterial biomass (log10 CFU mL−1) at T0 and after 7 days (TF) in the bioaugmentation experiment with Pseudomonas putida AE015451 in MSM and STWW samples at different experimental conditions
| Treatments . | MSM . | STWW . | |||
|---|---|---|---|---|---|
| T0 . | TF . | T0 . | TF . | ||
| Control | – | 8.02 ± 0.04 b | 8.83 ± 0.33 a | 7.70 ± 0.33 b | 8.94 ± 0.37 a |
| PCP (mg L−1) | 30 | 2.24 ± 0.05 Ab | 8.64 ± 0.98 Aa | 7.90 ± 0.13 Aa | 7.85 ± 0.15 Ba |
| 50 | 2.61 ± 0.98 Ab | 7.67 ± 1.09 Aa | 8.00 ± 0.25 Aa | 8.64 ± 0.33 Aa | |
| 70 | 2.89 ± 1.03 Ab | 6.32 ± 1.92 Aa | 7.80 ± 1.03 Aa | 7.96 ± 0.14 Ba | |
| 100 | 2.20 ± 0.93 Ab | 5.51 ± 1.10 ABa | 8.10 ± 0.98 Aa | 8.48 ± 0.45 Aa | |
| 200 | 2.23 ± 0.92 Ab | 4.55 ± 1.09 Ba | 7.54 ± 0.48 Aa | 8.27 ± 0.33 ABa | |
| 300 | 2.14 ± 1.03 Ab | 4.20 ± 1.10 Ba | 7.70 ± 0.66 Aa | 8.55 ± 0.33 Aa | |
| Fe (mg L−1) + 30 mg L−1 PCP | 5 | 8.61 ± 0.01 Aa | 8.86 ± 0.47 Aa | 7.70 ± 0.33 Aa | 8.21 ± 0.29 Ba |
| 10 | 8.41 ± 0.04 Ba | 8.79 ± 0.25 Aa | 7.80 ± 0.13 Aa | 7.83 ± 0.46 Ba | |
| 20 | 8.37 ± 0.01 Ba | 8.38 ± 0.98 Aa | 8.20 ± 0.36 Ab | 8.61 ± 0.15 Aa | |
| Inoculum (mL) + 30 mg L−1 PCP | 0.5 | 6.90 ± 0.02 Cb | 7.76 ± 0.02 Ca | 7.70 ± 0.15 Ab | 8.45 ± 0.16 Aa |
| 1 | 7.09 ± 0.02 Bb | 8.98 ± 0.01 Aa | 7.80 ± 1.03 Aa | 7.63 ± 0.46 Ba | |
| 2 | 8.02 ± 0.05 Aa | 8.06 ± 1.98 Aa | 8.10 ± 0.98 Aa | 8.66 ± 0.27 Aa | |
| pH + 30 mg L−1 PCP | 4 | 3.66 ± 0.92 Bb | 5.65 ± 1.03 Ca | 7.70 ± 0.33 Aa | 7.54 ± 0.58 Aa |
| 5 | 3.01 ± 1.09 Bb | 8.70 ± 0.92 Aa | 7.80 ± 0.24 Aa | 8.70 ± 0.70 Aa | |
| 6.3 | 3.06 ± 1.02 Bb | 9.24 ± 0.35 Aa | 7.70 ± 1.04 Aa | 8.83 ± 1.25 Aa | |
| 7 | 7.72 ± 1.04 Aa | 6.80 ± 0.78 Bb | 7.60 ± 0.97 Aa | 7.80 ± 0.98 Aa | |
| 8 | 7.91 ± 1.03 Aa | 4.22 ± 0.98 Cb | 8.09 ± 0.20 Aa | 8.17 ± 1.10 Aa | |
| Treatments . | MSM . | STWW . | |||
|---|---|---|---|---|---|
| T0 . | TF . | T0 . | TF . | ||
| Control | – | 8.02 ± 0.04 b | 8.83 ± 0.33 a | 7.70 ± 0.33 b | 8.94 ± 0.37 a |
| PCP (mg L−1) | 30 | 2.24 ± 0.05 Ab | 8.64 ± 0.98 Aa | 7.90 ± 0.13 Aa | 7.85 ± 0.15 Ba |
| 50 | 2.61 ± 0.98 Ab | 7.67 ± 1.09 Aa | 8.00 ± 0.25 Aa | 8.64 ± 0.33 Aa | |
| 70 | 2.89 ± 1.03 Ab | 6.32 ± 1.92 Aa | 7.80 ± 1.03 Aa | 7.96 ± 0.14 Ba | |
| 100 | 2.20 ± 0.93 Ab | 5.51 ± 1.10 ABa | 8.10 ± 0.98 Aa | 8.48 ± 0.45 Aa | |
| 200 | 2.23 ± 0.92 Ab | 4.55 ± 1.09 Ba | 7.54 ± 0.48 Aa | 8.27 ± 0.33 ABa | |
| 300 | 2.14 ± 1.03 Ab | 4.20 ± 1.10 Ba | 7.70 ± 0.66 Aa | 8.55 ± 0.33 Aa | |
| Fe (mg L−1) + 30 mg L−1 PCP | 5 | 8.61 ± 0.01 Aa | 8.86 ± 0.47 Aa | 7.70 ± 0.33 Aa | 8.21 ± 0.29 Ba |
| 10 | 8.41 ± 0.04 Ba | 8.79 ± 0.25 Aa | 7.80 ± 0.13 Aa | 7.83 ± 0.46 Ba | |
| 20 | 8.37 ± 0.01 Ba | 8.38 ± 0.98 Aa | 8.20 ± 0.36 Ab | 8.61 ± 0.15 Aa | |
| Inoculum (mL) + 30 mg L−1 PCP | 0.5 | 6.90 ± 0.02 Cb | 7.76 ± 0.02 Ca | 7.70 ± 0.15 Ab | 8.45 ± 0.16 Aa |
| 1 | 7.09 ± 0.02 Bb | 8.98 ± 0.01 Aa | 7.80 ± 1.03 Aa | 7.63 ± 0.46 Ba | |
| 2 | 8.02 ± 0.05 Aa | 8.06 ± 1.98 Aa | 8.10 ± 0.98 Aa | 8.66 ± 0.27 Aa | |
| pH + 30 mg L−1 PCP | 4 | 3.66 ± 0.92 Bb | 5.65 ± 1.03 Ca | 7.70 ± 0.33 Aa | 7.54 ± 0.58 Aa |
| 5 | 3.01 ± 1.09 Bb | 8.70 ± 0.92 Aa | 7.80 ± 0.24 Aa | 8.70 ± 0.70 Aa | |
| 6.3 | 3.06 ± 1.02 Bb | 9.24 ± 0.35 Aa | 7.70 ± 1.04 Aa | 8.83 ± 1.25 Aa | |
| 7 | 7.72 ± 1.04 Aa | 6.80 ± 0.78 Bb | 7.60 ± 0.97 Aa | 7.80 ± 0.98 Aa | |
| 8 | 7.91 ± 1.03 Aa | 4.22 ± 0.98 Cb | 8.09 ± 0.20 Aa | 8.17 ± 1.10 Aa | |
Different capital letters show significant differences among the different experimental conditions (Fe concentration, inoculum volume, PCP concentration, and pH) in the sampling time. The different lowercase letter shows differences between the two sampling times (T0 and TF).