This study evaluated antibiotic degradation and biogas production during anaerobic digestion of dairy manure contained two common veterinary antibiotics at 37 °C. After 18 days of digestion, the concentration of chlortetracycline (CTC) decreased more than 80% regardless of the initial CTC concentration. The concentration of cefazolin (CEZ) decreased from 10 to 0.08 mg/L in 6 days. Less than 50 mg/L CTC and 10 mg/L CEZ had negligible impact on biogas production during anaerobic digestion process. The result showed that the anaerobic digestion has a potential to degrade antibiotic residues in livestock manure.

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