Salt water from the Tagus Estuary has been identified in the influent at Barreiro/Moita WWTP, Portugal. The saltwater intrusion occurs during high tide levels in the estuary throughout damaged sections and direct vectors in the sewer network, changing the wastewater characteristics impacting the WWTP processes. This work has designed a methodology to assess from which tide level in the Tagus Estuary saltwater intrusion occurs in the sewer network by measuring WWTP influent's electric conductivity (EC). The methodology identifies saltwater intrusion for tide levels higher than 3.10 m, increasing significantly for tide levels higher than 4.00 m. During this study, 86% of the days registered at least one high tide level higher than 3.10 m, and 8% higher than 4.00 m, demonstrating a considerable occurrence of saltwater intrusion in the system with a tendency to increase due to the mean sea level rise registered in the Tagus Estuary. To preventing it, it is necessary to map infrastructures and assess the most critical points.