Treatment of waste cutting fluid by coagulation-anaerobic hydrolysis (with or without cosubstrate glucose)-aeration process

A coagulation-anaerobic hydrolysis-aeration process was used to treat the waste cutting fluid, which was discharged from a metalworking plant, and the operating conditions of each unit were optimized in this paper. The results showed that 9 g/L polyaluminum chloride and 0.3 g/L cationic polyacrylamide were added in the coagulation stage, the TOC was removed by 78.94% and the BOD5/COD of the waste cutting fluid increased from 0.046 to 0.312 before and after coagulation. The coagulation effluent was further treated by anaerobic hydrolysis-aeration, and the TOC removal efficiencies of the biological process and the whole chemical-biological process were 92.77% and 98.48% respectively. Adding glucose as a cosubstrate into the anaerobic hydrolysis can improve the TOC removal efficiency; when the TOC content ratio of coagulation effluent to glucose solution was 7:3, the TOC removal efficiencies of the biological process and the chemical-biological process reached 97.16% and 99.40%, and the total oil removal efficiency of the whole process reached 99.99%. The effluent quality parameters of the coagulation-anaerobic hydrolysis (with cosubstrate glucose)-aeration process met the Class C limits specified in the Wastewater Quality Standards for Discharge to Municipal Sewers (GB/T 31962-2015); that is, the effluent COD, TN, TP and total oil were below 300 mg/L, 25 mg/L, 5 mg/L and 110 mg/L, respectively, and the effluent pH was between 6.5–9.5.


INTRODUCTION
Cutting fluid is a kind of machining fluid used to clean, cool and lubricate tools during the metal cutting and grinding process, it helps to improve the life cycle and function of machining equipment (Yan et al. 2016). After use, the cutting fluid will lose the lubrication and cooling performance, and then need to be replaced periodically, which will eventually generate a large amount of cutting fluid wastewater. Waste cutting fluid has a high organic and oil content and poor biodegradability, which is extremely harmful to the environment and human health when it is discharged directly (Srikant et al. 2009;Feng et al. 2017). In recent years, the treatment methods for waste cutting fluid have been reported, mainly including electrochemical methods (Kobya et al. 2008;Chachou et al. 2015;Chawaloesphosiya et al. 2015;Xiong et al. 2020), advanced oxidation methods (Sánchez-Oneto et al. 2007;Seo et al. 2007;Liu et al. 2017;Zhang et al. 2017;Su et al. 2018) and membrane separation methods (Chen et al. 2019;Popovićet al. 2019). However, these methods often require high-cost drugs and expensive equipment, beyond the economic capacity of the ordinary factory, so it is difficult to apply in practical engineering (Chiang et al. 2001;Emami-Meibodi et al. 2016;Intini & Liberti 2016;Boczkaj & Fernandes 2017).
Anaerobic biological treatment has low energy consumption and sludge production, and can undertake high organic loads. In addition, aerobic organisms have a fast multiplication rate and short acclimation time. Therefore, some researchers have found that a combined anaerobic-aerobic process is a feasible way to treat industrial wastewater (Alayu & Yirgu 2018;Sun et al. 2018;Dhaouefi et al. 2019). However, the organics and oil in waste cutting fluid, which can reach 10 4 -10 5 mg/L, are much higher than those of ordinary industrial wastewater. So, in order to remove a large amount of emulsified oil and some organic matter, the coagulation method was planned to pretreat the waste cutting fluid and then the biological treatment process was considered. Compared with methanogenic bacteria, hydrolytic acidifying bacteria are less sensitive to temperature, pH and toxic substances. Hence, the anaerobic metabolism of microorganisms is controlled in the hydrolysis acidification stage, which will not only shorten the operation time of the treatment process, but also be more suitable for the treatment of refractory organic wastewater such as waste cutting fluid (Perez et al. 2006;Shi 2011). Therefore, the waste cutting fluid was treated by the coagulation-anaerobic hydrolysis-aeration process, and the operating conditions of each treatment unit were optimized, and meanwhile the role of glucose as a cosubstrate of the waste cutting fluid in anaerobic hydrolysis unit was investigated.

Wastewater characteristics and standard limits
The waste cutting fluid was taken from an aluminum metalworking plant in Tianjin, and its color was opaque milky white. The main characteristics of the waste cutting fluid and corresponding limits specified in the Wastewater Quality Standards for Discharge to Municipal Sewers (GB/T 31962-2015) are shown in Table 1.

Analytical methods
The dissolved oxygen (DO) and pH were measured in situ by the portable DO meter (WTW FDO925) and pH meter (WTW SenTix940-3). The TOC, COD, BOD 5 , TN, TP, total oil and total dissolved solid were analyzed according to Chinese standard methods (CEPB 2002).

Screening coagulant
Six coagulants including calcium chloride (CaCl 2 ), polymerized ferric sulfate (PFS), polymerized aluminum chloride (PAC), ferric chloride (FeCl 3 ), iron sulfate (Fe 2 (SO 4 ) 3 ) and aluminum sulfate (Al 2 (SO 4 ) 3 ) were selected for coagulation pretreatment with different dosages (3 g/L, 6 g/L, 9 g/L, 12 g/L, 15 g/L, 18 g/L). 350 ml waste cutting fluid and the coagulant were put into a 500 ml beaker successively, and then the beaker was placed on the ER4-6 coagulation experiment mixer with quick stirring at 200 rpm for 4 minutes, then slow stirring at 50 rpm for 5 minutes. Finally, the beaker with the mixture was settled for 2 hours. The supernatant pH 9.4-9.7 6.5-9.5 6.5-9.5 6.5-9.5 Total dissolved solids (mg/L) / 1,500 2,000 2,000 sample was taken from the beaker and used to analyze TOC and Zeta potential. The optimum dosages of the six coagulants and the best coagulant were determined according to Zeta potential, TOC removal efficiency, pH and B/C.

Determination of the best coagulation conditions
Four 500 ml beakers were taken and 350 ml waste cutting fluid was put into each beaker, and then the four beakers were added with the best coagulant of 3 g/L, 6 g/L, 9 g/L and 12 g/L respectively. The four beakers were placed on the ER4-6 coagulation experiment mixer. After rapid stirring at 200 rpm for 4 minutes, 0.3 g/L cationic polyacrylamide (CPAM) were added into each beaker, and then slowly stirred at 50 rpm for 5 minutes with static settling for 2 hours. The supernatant sample was taken from the beaker and used to analyze TOC, pH and B/C, and then the optimal dosage of the best coagulant with 0.3 g/L coagulant aid was determined. The optimal dosage of the best coagulant was fixed, and the CPAM dosage (g/L), the rapid stirring speed (r/min), and rapid stirring time (min) were selected for three factors and three levels L 9 (3 4 ) orthogonal design to determine the optimal coagulation conditions. Under the optimal coagulation conditions, the TOC, COD, B/C TN, TP, total oil and pH of coagulation effluent were measured.

Acclimation of the biological reactors
Both anaerobic hydrolysis and aerobic reactors were cylindrical and made of plexiglass. The total volume and working volume of each reactor were 5 L and 3.5 L respectively. Both anaerobic hydrolysis and aerobic reactors were equipped with a stirrer to mix the activated sludge and waste cutting fluid. Moreover, an aerator was set in the aerobic reactor to oxygenate and keep DO not less than 3 mg/L. The schematic and physical diagrams of the biological treatment apparatuses are shown in Figure 1(a) and 1(b) respectively. The influent compositions of anaerobic hydrolysis reactor during each biological treatment stage are listed in Table 2.  The TOC concentration of glucose solution was similar to that of coagulation effluent, about 3,600 mg/L. In each phase, the anaerobic hydrolysis reactor effluent of the day was injected into the aerobic reactor as the aerobic reactor influent. The seed sludge was acquired from the secondary sedimentation tank of a municipal WWTP in Tianjin. The anaerobic hydrolysis and aerobic reactors were operated intermittently (namely daily running 22 h, static settling 1.5 h, drainage and inflow 0.5 h). 500 ml supernatant was discharged from the anaerobic hydrolysis reactor, and then 500 ml synthetic wastewater or coagulation effluent were added into the anaerobic hydrolysis reactor, and the ratio of coagulation effluent to glucose solution in synthetic wastewater gradually increased during acclimation, which is shown in Table 2. 500 ml supernatant was discharged from the aerobic reactor too, and then the 500 ml anaerobic hydrolysis effluent of the day was added into the aerobic reactor.
At the end of stable operation phase, the anaerobic hydrolysis and aerobic reactors were continuously operated for 24 hours. 50 ml of mixture was extracted from the anaerobic hydrolysis reactor and aerobic reactor respectively every 3 hours, and after static settling the supernatant TOC of each 50 ml mixture was measured, and then the optimum running times of biological reactors were determined by the TOC variations with time.

Cometabolism experiment
After the stable operation phase, glucose was studied as the cosubstrate. The influent of the cometabolism phase is listed in Table 2. Except that the running time was adjusted to the optimum conditions within the last 7 days of the cometabolism phase, other experimental conditions were the same as above.

Screening coagulant
Since the chloride and sulfate ions in some coagulants may affect the accuracy of COD measurement, TOC was used as the main parameter to characterize the organic matter treatment results of the waste cutting fluid. As shown in Figure 2(a), six coagulants had different treatment effects on waste cutting fluid. In the beginning, the coagulation effluent TOC decreased with the increase of coagulant dosages. When the coagulants were overdosed, the removal effect of TOC became worse, which may be due to the restabilization of the coagulant (Zhang et al. 2007).
The stability of colloidal particles is related to their Zeta potential. The particles with higher absolute value of zeta potential have larger repulsive force, are not easy to settle and are relatively stable; the particles with lower absolute value of zeta potential have smaller repulsive force, and are more likely to settle. When the Zeta potential is 0, the particles are the most unstable and easiest to settle (Cheng et al. 2012). Therefore, Zeta potential variations of six coagulants at different dosages were also measured to determine the best coagulant. As shown in Figure 2(b), the original Zeta potential value of the waste cutting fluid was À25.8 mV, which was mainly caused by a large amount of anionic surfactant in the waste cutting fluid. During coagulation, the positively charged coagulant hydrolysate gradually neutralized the negatively charged particles, and then the repulsive force between particles was reduced. Therefore, the absolute values of Zeta potential decreased with the increasement of the coagulant dosages. When the coagulant dosages increased to a certain value, the change of the Zeta potential values tended to be slow, which was consistent with the TOC variations.
According to the variations of TOC and Zeta potential, the optimal dosage of each coagulant was determined. Meanwhile the TOC removal efficiency, pH, and B/C of the coagulation effluent at the optimal dosage of each coagulant are listed in Table 3. Table 3, coagulation pretreatment changed the pH value and biodegradability of waste cutting fluid. However, CaCl 2 is a strong acid-strong base salt, and it is ionized after being dissolved in wastewater, so it had the least effect on the coagulation effluent pH among the six coagulants.

As shown in
However, Fe 2 (SO 4 ) 3 , FeCl 3 and Al 2 (SO 4 ) 3 coagulants are strong acid-weak base salts, which perform acidity after being dissolved in wastewater, and their dosages were large, so they had a great impact on the coagulation effluent pH. In addition, the proton with positive charge neutralized the negative charge on the particle, which lowered the absolute value of the Zeta potential, promoted particle destabilization and enhanced coagulation. Therefore, the demulsification performances of the above three coagulants were better than that of CaCl 2 .
During the hydrolysis of PAC and PFS, a large number of hydroxide polymers or complex ions are generated, thus PAC and PFS not only have the same salting out effect as CaCl 2 but also the adsorption effect of polymer coagulant (Tang & Luan 1997;Chawaloesphonsiya et al. 2018). However, the waste cutting fluid contains a lot of polycyclic aromatic hydrocarbons and heterocyclic compounds, and most of these organics have molecular weights greater than 100 ku. Under the same dosage, PAC has a better effect than PFS on the removal of macromolecular organics (Han et al. 2018). Therefore, B/C of PAC coagulation effluent was higher than PFS coagulation effluent, and PAC had the best effect on the removal of organics and improvement of biodegradability.
As shown in Figure 2(a), when the dosage of each coagulant was not more than 15 g/L, PAC had the best demulsification performance under the same dosage. As shown in Figure 2(a) and Table 3, when the dosage of PAC was 15 g/L, the TOC removal efficiency reached 79.8% and the B/C of the waste cutting fluid increased to 0.491, but the coagulation effluent pH was 6.301, which was lower than the optimal pH range (i.e., 6.5-8.5) for microbial growth and not conducive to subsequent biological treatment. In summary, PAC was selected as the best coagulant for subsequent experiments, and the dosage of PAC was preferably less than 15 g/L.

Optimization experiment of coagulation conditions
In order to reduce the cost of coagulant, a small amount of coagulant aid was used to reduce the PAC dosage.
As shown in Figure 3(a), under different PAC dosages, the removal efficiencies of TOC with CPAM were improved by 3% ∼ 6% compared with that without CPAM. When 9 g/L PAC and 0.3 g/L CPAM were added, the TOC removal efficiency was 78.3%. When only 12 g/L PAC was added, namely without CPAM, the TOC removal efficiency was 76.0%. Therefore, adding 0.3 g/L CPAM could reduce the dosage of PAC by 3 g/L and the cost of wastewater treatment by 3.9 CNY/m 3 (0.6 USD/m 3 ; the China Yuan (CNY) cost was converted to US Dollars (USD) at the rate of 1 USD ¼ 6.5 CNY, and the reagents prices are listed in the section Estimation of reagent cost), and meanwhile the removal efficiency of TOC would not be decreased. As shown in Figure 3(b), when 9 g/L PAC and 0.3 g/L CPAM were added, the coagulation effluent pH was 7.808 and B/C was 0.312, both of which met the growth conditions of microorganisms. Hence the dosage of PAC was selected as 9 g/L when CPAM was used as the coagulant aid. PAC dosage was fixed at 9 g/L. The CPAM dosage, rapid stirring speed, and rapid stirring time were selected for three factors and three levels L 9 (3 4 ) orthogonal design, as shown in Table 4. The results of orthogonal design are shown in Table 5.
According to the orthogonal experiment results, the factors affecting the coagulation performance were ranked as B . A . C, namely rapid stirring speed . CPAM dosage . rapid stirring time. The optimal coagulation conditions were B3A2C2, namely 0.3 g/L CPAM, and quick stirring for 5 minutes at 450 r/min. The verification experiment was carried out under the optimal coagulation conditions, namely adding 9 g/L PAC and 0.3 g/L CPAM, quick stirring for 5 min at 450 r/min, slow stirring for 5 min at 50 r/min, and then settling for 2 h. The verification experiment results are shown in Table 6.

Acclimation of the biological reactors
The influent and effluent TOC and TOC removal efficiencies of the anaerobic hydrolysis and aerobic reactors are shown in Figure 4.
The domestication process was implemented for five phases (I ∼ V), a total of 56 days, and each phase ran for 7 or 14 days. The proportion of coagulation effluent in the influent of anaerobic hydrolysis reactor gradually increased at each phase.
As shown in Figure 4(a), in the phase of domestication I, 500 ml glucose solution, seed sludge and trace element solution were fed into the anaerobic hydrolysis reactor on the first day, and tap water was used to make up the working volume until the total volume of mixed liquid reached 3.5 L. Therefore, because of dilution effect, the effluent TOC was low and the TOC removal efficiency was high on the first day. With the continuous  inflow of the influent every day, organic matter was continuously accumulated in reactor, and the dilution effect was weakened day by day, and then the effluent TOC gradually increased and TOC removal efficiency decreased.
In the phase of domestication II, when the coagulation effluent was added as a part of the anaerobic hydrolysis reactor influent, because microorganisms did not adapt to the coagulation effluent of waste cutting fluid, the effluent TOC increased rapidly and TOC removal efficiency dropped sharply. During domestication phases III and IV, because microorganisms gradually adapted to the coagulation effluent of waste cutting fluid, the increase of effluent TOC and the decrease of TOC removal efficiency slowed down, even though the proportion of coagulation effluent in the influent increased. At the end of domestication phase II, the anaerobic hydrolysis effluent became clear. After domestication phase IV, the activated sludge in the anaerobic hydrolysis reactor became flocculent from the initial paste, and settled better. During domestication phase V, the effluent TOC of anaerobic hydrolysis reactor no longer increased, even though all anaerobic hydrolysis influent was the coagulation effluent. At the end of domestication phase V, the effluent TOC and TOC removal efficiency of the anaerobic hydrolysis reactor were about 2,700 mg/L and 24.19% respectively. The aerobic reactor influent was the anaerobic hydrolysis effluent of the day. As shown in Figure 4(b), in the phase of domestication I, the anaerobic hydrolysis effluent TOC was increased, so the effluent TOC and TOC removal efficiency of the aerobic reactor slightly increased and decreased, respectively. As the microorganisms gradually adapt to the waste cutting fluid from domestication phase II to domestication phase IV, the aerobic  reactor effluent TOC dropped gradually and TOC removal efficiency rose step by step, even though the proportion of coagulation effluent in the anaerobic hydrolysis influent increased. However, in the phase of domestication V, due to the influent of anaerobic hydrolysis reactor being 100% coagulation effluent of waste cutting fluid, the effluent TOC and TOC removal efficiency of the aerobic reactor were greater and lower than those of domestication IV respectively. At the end of domestication phase V, the aerobic reactor effluent TOC was about 200 mg/L. In the stable operation phase, as in domestication phase V, all influent of the anaerobic hydrolysis reactor was coagulation effluent. As shown in Figure 4(a), in the first 21 days of the stable operation phase, the anaerobic hydrolysis effluent TOC was similar to that in domestication phase V. In the next 42 days of stable operation phase, because microorganisms adapt to the coagulation effluent better, the anaerobic hydrolysis effluent TOC dropped to about 2,200 mg/L, and the TOC removal efficiency rose to about 38.8%, and the B/C of the anaerobic hydrolysis effluent was increased to 0.47. Therefore, in the anaerobic hydrolysis reactor, the refractory organics could be hydrolyzed into small molecule biodegradable organics and part of the readily biodegradable organics was likely to be used for anaerobic methane production.
As shown in Figure 4(b), the effluent TOC and TOC removal efficiency of the aerobic reactor fluctuated less although the influent of the aerobic reactor changed greatly, which indicated that the aerobic reactor removed the organic matters effectively and had a good resistance to shark load. The aerobic reactor effluent TOC in the stable operation phase was about 260 mg/L, and the TOC removal efficiency of the aerobic reactor was about 88.18%.
The TOC removal efficiency of the biological treatment process, namely the anaerobic hydrolysis-aeration process, was 92.77%, and the TOC removal efficiency of the entire treatment process, namely the coagulationanaerobic hydrolysis-aeration process, reached 98.48%.
At the end of the stable operation phase, within 24 h, the effluent TOC of the anaerobic hydrolysis and aerobic reactors were measured respectively every 3 h to investigate the effect of the running time on the organics removal in the biological treatment reactors.
As shown in Figure 5, the effluent TOC of the anaerobic hydrolysis and aerobic reactors had an obvious decrease in the first 12 hours, which indicated that the biodegradation was almost complete when the reactors ran for 12 hours. In the full-scale treatment plant, if the biological treatment reactor runs longer than its needs, it will not only increase the reactor volume but also increase the operation and investment costs. Therefore, the optimal running time for both anaerobic hydrolysis and aerobic reactors was 12 hours.

Cometabolism experiment
When the waste cutting fluid was treated by the coagulation-anaerobic hydrolysis-aeration process, in the stable operation phase, the effluent TOC of the above process was about 260 mg/L, which failed to meet the organics limit listed in Table 1. However, it was found that the effluent TOC in the domestication phase IV was lower than that in domestication phase II, III, V and stable operation phase. In the domestication phase IV, the TOC content ratio of coagulant effluent to glucose solution in the anaerobic hydrolysis influent was 7:3, which indicated that under the same influent organic load, adding some readily biodegradable organics was conducive to the refractory organic's removal. So, after the stable operation phase, glucose solution was added into the anaerobic hydrolysis influent again to verify the effect of cometabolism.
As shown in Figure 4, at the end of cometabolism phase, compared with the stable operation phase, the effluent TOC of the anaerobic hydrolysis reactor decreased from about 2,200 mg/L to about 2,000 mg/L, and the effluent TOC of the aerobic reactor decreased from about 260 mg/L to about 100 mg/L. If there was no cometabolism of glucose, and all the glucose was degraded in biological treatment reactors, the effluent TOC of aerobic reactor should be about 180 mg/L (namely, 260 Â 0.7 ¼ 182 mg/L) due to the dilution of coagulation effluent. Therefore, that indicated the glucose could be used as a cosubstrate in the biological treatment process of waste cutting fluid.
The waste cutting fluid was treated by the coagulation-anaerobic hydrolysis (with cosubstrate glucose) -aeration process, and both anaerobic hydrolysis and aerobic reactors were operated for 14 hours in one operational period (namely running 12 h, static settling 1.5 h, drainage and inflow 0.5 h), and then the effluent characteristics at the end of cometabolism phase were measured and shown in Table 7.
As shown in Table 7, when the waste cutting fluid was treated by the coagulation-anaerobic hydrolysis -aeration process and the glucose as a cosubstrate was added into the anaerobic hydrolysis reactor with the TOC content ratio of coagulation effluent to glucose solution was 7:3, the main pollutants in the aerobic reactor effluent met the Class C limits specified in the Wastewater Quality Standards for Discharge to Municipal Sewers (GB/T 31962-2015), and the removal efficiencies of TOC, COD and total oil were all over 99%.

CONCLUSION
Compared with CaCl 2 , Fe 2 (SO 4 ) 3 , FeCl 3 , PFS and Al 2 (SO 4 ) 3 , PAC had the best effect on the removal of organics and could improve the biodegradability of the waste cutting fluid. The CPAM was used as coagulant aid to reduce the dosage of PAC. When the dosages of PAC and CPAM were 9 g/L and 0.3 g/L, after the coagulation pretreatment, the TOC removal efficiency was 78.94%, the total oil removal efficiency reached 99.83%, and the B/C value of the coagulation effluent increased to above 0.3.
The organic matters in the coagulation effluent could be removed by the anaerobic hydrolysis-aeration treatment process, and the biodegradability of wastewater was improved by anaerobic hydrolysis reactor, and the B/C of anaerobic hydrolysis effluent was increased to 0.47. The TOC removal efficiencies of the biological process and the whole chemical-biological process were 92.77% and 98.48% respectively.
Adding glucose as a cosubstrate into the anaerobic hydrolysis unit promoted the degradation of the refractory organics. When the TOC content ratio of coagulation effluent to glucose solution was 7:3, the effluent TOC of aerobic reactor was reduced to about 100 mg/L. Nevertheless, the average effluent TOC of aerobic reactor was about 260 mg/L without glucose as a cosubstrate. The effluent COD, TN, TP, total oil and pH of the coagulation-anaerobic hydrolysis (with cosubstrate glucose)-aeration process met the Class C limits specified in the Wastewater Quality Standards for Discharge to Municipal Sewers (GB/T 31962-2015).
As the quality of waste cutting fluid produced by different metalworking plants would be different, the above treatment process could be used for the treatment of different waste cutting fluids, but the dosages of PAC and CPAM, and coagulation conditions need to optimized again.

ACKNOWLEDGEMENT
This research work was financially supported by the National Natural Science Foundation of China (No. 51678388).