The effects of chlorine and pipe material on biofilm development and structure were studied in a model reclaimed water distribution system using annular reactors (ARs). The initial chlorine dose of influents in ARs with polycarbonate pipes and cast iron pipes was 3.59 and 10.15 mg/L, respectively, which could maintain the reclaimed water quality (HPC 102–103 CFU/mL). The chlorine and pipe material influenced the bacterial community. Biofilms formed faster and to a greater extent in ARs with cast iron coupons (HPC 107 CFU/cm2) compared to those with polycarbonate coupons (HPC 105 CFU/cm2). Using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis, it was found that Gammaproteobacteria were prevalent in all cases with polycarbonate pipes, whereas with cast iron pipes microbial populations were affiliated with the phylum Proteobacteria, including the classes Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Deltaproteobacteria, followed by the phyla Bacteroidetes and uncultured bacterium.
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Research Article|
May 01 2012
Effects of chlorine and pipe material on biofilm development and structure in a reclaimed water distribution system Available to Purchase
Haibo Wang;
Haibo Wang
1State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China
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Xuexiang Hu;
Xuexiang Hu
1State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China
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Chun Hu
1State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China
E-mail: [email protected]
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Water Supply (2012) 12 (3): 362–371.
Article history
Received:
July 22 2011
Accepted:
January 05 2012
Citation
Haibo Wang, Xuexiang Hu, Chun Hu; Effects of chlorine and pipe material on biofilm development and structure in a reclaimed water distribution system. Water Supply 1 May 2012; 12 (3): 362–371. doi: https://doi.org/10.2166/ws.2012.005
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